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Immunocytochemistry in Cytology

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Abstract

In several reviews (Nadji 1980; Walts and Said 1983; Koss 1984; Chess and Hajdu 1986) the considerable value of the application of immunocytochemical methods in cytology (ICC) has been indicated. However, despite the high number of reports focused on the application of ICC as a diagnostic tool it did not have the enormous practical impact of immunohistochemistry. This may be due to methodological difficulties and the existence of a vast number of different protocols for fixation and storage and the different immunocytochemical techniques used for cytological preparations. Because of inaccuracies in the descriptions of fixation and processing of cell preparations in many papers on ICC, a comparison of data obtained by different authors is impossible or at least questionable.

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References

  • Chess Q, Hajdu SI (1986) The role of immunoperoxidase staining in diagnostic cytology. Acta Cytol (Baltimore) 30:1–7

    CAS  Google Scholar 

  • Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonalds S, Pulfords KAF, Mason DY (1984) Immunoenzymatic labeling of monoclonal antibodies using complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229

    Article  PubMed  CAS  Google Scholar 

  • Dalquen P, Bittel D, Gudat F, Overbeck J, Heitz PH (1986) Combined immunoreaction and Papanicolaou’s stain on cytological smears. Pathol Res Pract 181: 50–54

    Article  PubMed  CAS  Google Scholar 

  • Denk H (1987) Immunohistochemical methods for the demonstration of tumor markers. In: Seifert G (ed) Morphological tumor markers. Springer, Berlin Heidelberg New York, pp 47–69 (Current topics in pathology, vol 77)

    Chapter  Google Scholar 

  • DeWaele M, DeMey J, Moeremans M, DeBrabander M, van Camp B (1983) Immunogold staining method for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies: its application to the enumeration of lymphocyte subpopulations. J Histochem Cytochem 31: 376–381

    Article  CAS  Google Scholar 

  • Erber WN, Pinching AJ, Mason DY (1984) Immunocytochemical detection of T and B cell populations in routine blood smears. Lancet I: 1042–1045

    Article  Google Scholar 

  • Faravelli A, Sironi M, Villa E, Radice F (1984) Immunoperoxidase study of cytologic smears in oral pemphigus. Acta Cytol (Baltimore) 28: 414–418

    CAS  Google Scholar 

  • Geddie WR, Bedard YC, Strawbridge HTG (1984) Medullary carcinoma of the thyroid in fine needle aspiration biopsies. Am J Clin Pathol 82: 552–558

    PubMed  CAS  Google Scholar 

  • Gosselin EJ, Dennett JC, Sorenson GD, Pettengill OS, Cate CC (1985) Immunocytochemical staining of cytocentrifuge prepared cultured cells: nonspecific staining and its elimination. Histochem J 17: 847–858

    Article  PubMed  CAS  Google Scholar 

  • Gower WR, McSweeney EM, Dyben TE, Fabri PJ (1985) Identification of gastrinproducing cells in cell cultures and smears: an avidin-biotin-peroxidase complex (ABC) technique. J Histochem Cytochem 33: 1087–1089

    Article  PubMed  CAS  Google Scholar 

  • Hsu SM, Raine L, Fanger H (1981) The use of avidin-biotin-peroxidase complex in immunperoxidase techniques. Am J Clin Pathol 75: 816–861

    PubMed  CAS  Google Scholar 

  • Judd MA, Britten KJM (1982) Tissue preparation for the demonstration of surface antigens by immunoperoxidase techniques. Histochem J 14: 747–753

    Article  PubMed  CAS  Google Scholar 

  • Keshgegian AA, Kline TS (1984) Immunoperoxidase demonstration of prostatic acid phosphatase in aspiration biopsy cytology (ABC). Am J Clin Pathol 82: 586–589

    PubMed  CAS  Google Scholar 

  • Kobayashi TK, Mizuhara S, Arai M, Sawaragi I (1985) Application of immunoperoxidase staining in the cytodiagnosis of herpes simplex keratitis. Diagn Cytopathol 1: 317–321

    Article  PubMed  CAS  Google Scholar 

  • Koss LG (1984) Cytochemistry (Editorial). Acta Cytol (Baltimore) 28: 353–355

    CAS  Google Scholar 

  • Leenen PJM, Jansen AMA, van Ewijk W (1985) Fixation parameters for immunohistochemistry. The effect of glutaraldehyde or paraformaldehyde fixation on the preservation of mononuclear phagocyte differentiation antigens. In: Bullock GR, Petrusz P (eds) Techniques in immunohistochemistry, vol III. Academic, NY, pp 1–24

    Google Scholar 

  • Li CY, Ziesmer SC, Yam LT, English MC, Janckila AJ (1984) Practical immunocytochemical identification of human blood cells. Am J Clin Pathol 81: 204–212

    PubMed  CAS  Google Scholar 

  • Nadji M (1980) The potential value of immunoperoxidase techniques in diagnostic cytology. Acta Cytol (Baltimore) 24: 442–447

    CAS  Google Scholar 

  • Ramaekers F, Haag D, Jap P, Vooijs PG (1984) Immunocytochemical demonstration of keratin and vimentin in cytologic aspirates. Acta Cytol (Baltimore) 28: 385–392

    CAS  Google Scholar 

  • Springall DR (1986) Immunocytochemistry in diagnostic cytology. In: Polak JM, van Noorden S (eds) Immunocytochemistry. Modern method and applications. Wright, Bristol, pp 547–567

    Google Scholar 

  • Szpak CA, Johnston WW, Lottich SC, Kufe D, Thor A, Schlom J (1984) Problems of reactivity of four novel monoclonal antibodies (B72.3, DF3, Bl.l and B6.2) with cells in human malignant and benign effusions. Acta Cytol (Baltimore) 28: 356–367

    CAS  Google Scholar 

  • Travis WD, Wold LE (1987) Immunoperoxidase staining of fine needle aspiration specimens previously stained by papanicolaou technique. Acta Cytol (Baltimore) 31: 517–520

    CAS  Google Scholar 

  • Van Ewijk W, van Soest PL, Verkerk A, Jongkind JF (1984) Loss of antibody to prefixed cells: fixation parameters for immunohistochemistry. Histochem J 16: 179–193

    Article  PubMed  Google Scholar 

  • Walts AE, Said JW (1983) Specific tumor markers in diagnostic cytology: immunoperoxidase studies of carcinoembryonic antigen, lysozymes and other tissue antigens in effusions, washes and expirates. Acta Cytol (Baltimore) 27: 408–416

    CAS  Google Scholar 

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© 1988 Springer-Verlag Berlin Heidelberg

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Höfler, H., Wirnsberger, G., Dinges, H.P. (1988). Immunocytochemistry in Cytology. In: Goerttler, K., Feichter, G.E., Witte, S. (eds) New Frontiers in Cytology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73596-7_22

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  • DOI: https://doi.org/10.1007/978-3-642-73596-7_22

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73598-1

  • Online ISBN: 978-3-642-73596-7

  • eBook Packages: Springer Book Archive

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