Abstract
In several reviews (Nadji 1980; Walts and Said 1983; Koss 1984; Chess and Hajdu 1986) the considerable value of the application of immunocytochemical methods in cytology (ICC) has been indicated. However, despite the high number of reports focused on the application of ICC as a diagnostic tool it did not have the enormous practical impact of immunohistochemistry. This may be due to methodological difficulties and the existence of a vast number of different protocols for fixation and storage and the different immunocytochemical techniques used for cytological preparations. Because of inaccuracies in the descriptions of fixation and processing of cell preparations in many papers on ICC, a comparison of data obtained by different authors is impossible or at least questionable.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chess Q, Hajdu SI (1986) The role of immunoperoxidase staining in diagnostic cytology. Acta Cytol (Baltimore) 30:1–7
Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonalds S, Pulfords KAF, Mason DY (1984) Immunoenzymatic labeling of monoclonal antibodies using complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219–229
Dalquen P, Bittel D, Gudat F, Overbeck J, Heitz PH (1986) Combined immunoreaction and Papanicolaou’s stain on cytological smears. Pathol Res Pract 181: 50–54
Denk H (1987) Immunohistochemical methods for the demonstration of tumor markers. In: Seifert G (ed) Morphological tumor markers. Springer, Berlin Heidelberg New York, pp 47–69 (Current topics in pathology, vol 77)
DeWaele M, DeMey J, Moeremans M, DeBrabander M, van Camp B (1983) Immunogold staining method for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies: its application to the enumeration of lymphocyte subpopulations. J Histochem Cytochem 31: 376–381
Erber WN, Pinching AJ, Mason DY (1984) Immunocytochemical detection of T and B cell populations in routine blood smears. Lancet I: 1042–1045
Faravelli A, Sironi M, Villa E, Radice F (1984) Immunoperoxidase study of cytologic smears in oral pemphigus. Acta Cytol (Baltimore) 28: 414–418
Geddie WR, Bedard YC, Strawbridge HTG (1984) Medullary carcinoma of the thyroid in fine needle aspiration biopsies. Am J Clin Pathol 82: 552–558
Gosselin EJ, Dennett JC, Sorenson GD, Pettengill OS, Cate CC (1985) Immunocytochemical staining of cytocentrifuge prepared cultured cells: nonspecific staining and its elimination. Histochem J 17: 847–858
Gower WR, McSweeney EM, Dyben TE, Fabri PJ (1985) Identification of gastrinproducing cells in cell cultures and smears: an avidin-biotin-peroxidase complex (ABC) technique. J Histochem Cytochem 33: 1087–1089
Hsu SM, Raine L, Fanger H (1981) The use of avidin-biotin-peroxidase complex in immunperoxidase techniques. Am J Clin Pathol 75: 816–861
Judd MA, Britten KJM (1982) Tissue preparation for the demonstration of surface antigens by immunoperoxidase techniques. Histochem J 14: 747–753
Keshgegian AA, Kline TS (1984) Immunoperoxidase demonstration of prostatic acid phosphatase in aspiration biopsy cytology (ABC). Am J Clin Pathol 82: 586–589
Kobayashi TK, Mizuhara S, Arai M, Sawaragi I (1985) Application of immunoperoxidase staining in the cytodiagnosis of herpes simplex keratitis. Diagn Cytopathol 1: 317–321
Koss LG (1984) Cytochemistry (Editorial). Acta Cytol (Baltimore) 28: 353–355
Leenen PJM, Jansen AMA, van Ewijk W (1985) Fixation parameters for immunohistochemistry. The effect of glutaraldehyde or paraformaldehyde fixation on the preservation of mononuclear phagocyte differentiation antigens. In: Bullock GR, Petrusz P (eds) Techniques in immunohistochemistry, vol III. Academic, NY, pp 1–24
Li CY, Ziesmer SC, Yam LT, English MC, Janckila AJ (1984) Practical immunocytochemical identification of human blood cells. Am J Clin Pathol 81: 204–212
Nadji M (1980) The potential value of immunoperoxidase techniques in diagnostic cytology. Acta Cytol (Baltimore) 24: 442–447
Ramaekers F, Haag D, Jap P, Vooijs PG (1984) Immunocytochemical demonstration of keratin and vimentin in cytologic aspirates. Acta Cytol (Baltimore) 28: 385–392
Springall DR (1986) Immunocytochemistry in diagnostic cytology. In: Polak JM, van Noorden S (eds) Immunocytochemistry. Modern method and applications. Wright, Bristol, pp 547–567
Szpak CA, Johnston WW, Lottich SC, Kufe D, Thor A, Schlom J (1984) Problems of reactivity of four novel monoclonal antibodies (B72.3, DF3, Bl.l and B6.2) with cells in human malignant and benign effusions. Acta Cytol (Baltimore) 28: 356–367
Travis WD, Wold LE (1987) Immunoperoxidase staining of fine needle aspiration specimens previously stained by papanicolaou technique. Acta Cytol (Baltimore) 31: 517–520
Van Ewijk W, van Soest PL, Verkerk A, Jongkind JF (1984) Loss of antibody to prefixed cells: fixation parameters for immunohistochemistry. Histochem J 16: 179–193
Walts AE, Said JW (1983) Specific tumor markers in diagnostic cytology: immunoperoxidase studies of carcinoembryonic antigen, lysozymes and other tissue antigens in effusions, washes and expirates. Acta Cytol (Baltimore) 27: 408–416
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Höfler, H., Wirnsberger, G., Dinges, H.P. (1988). Immunocytochemistry in Cytology. In: Goerttler, K., Feichter, G.E., Witte, S. (eds) New Frontiers in Cytology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73596-7_22
Download citation
DOI: https://doi.org/10.1007/978-3-642-73596-7_22
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73598-1
Online ISBN: 978-3-642-73596-7
eBook Packages: Springer Book Archive