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New Frontiers in Cytology pp 13–21Cite as

In Situ Hybridization: An Overview

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Abstract

In situ hybridization procedures allow the detection and localization of specific nucleic acid sequences in microscopic preparations. In combination with immunocytochemistry, they make it possible to study the topological relation between gene and gene products. The method stems from the fact that two strands in a DNA double helix or a DNA-RNA duplex can be separated by denaturation and then reannealed (hybridized) with labeled complementary strands under conditions which favor specific duplex formation. A labeled DNA or RNA sequence (probe) is used, to localize the gene sequences of interest (the target sequence).

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Authors
  1. M. van der Ploeg
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  2. A. K. Raap
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Editor information

Editors and Affiliations

  1. Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-6900, Heidelberg, Federal Republic of Germany

    Klaus Goerttler

  2. Institut für Vergleichende und Experimentelle Pathologie der Universität, Im Neuenheimer Feld 220-221, D-6900, Heidelberg, Federal Republic of Germany

    Georg E. Feichter

  3. Abteilung Innere Medizin, Diakonissenhospital, D-7500, Karlsruhe, Federal Republic of Germany

    Siegfried Witte

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© 1988 Springer-Verlag Berlin Heidelberg

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van der Ploeg, M., Raap, A.K. (1988). In Situ Hybridization: An Overview. In: Goerttler, K., Feichter, G.E., Witte, S. (eds) New Frontiers in Cytology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73596-7_2

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  • DOI: https://doi.org/10.1007/978-3-642-73596-7_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73598-1

  • Online ISBN: 978-3-642-73596-7

  • eBook Packages: Springer Book Archive

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