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Structure of the Surface Glycoprotein from Halobacterium volcanii as Revealed by Electron Microscopy

  • M. Kessel
  • I. Wildhaber
  • S. Cohen
  • W. Baumeister
Conference paper

Abstract

The cell surfaces of Halobacteria are seen by shadow casting to be composed of a hexagonal lattice of morphological units with a centre-to-centre spacing of approximately 15 nm. Preparations of virtually salt-free envelopes from Halobacterium volcanii, isolated from the Dead Sea, can be obtained by freezing and thawing the cells, treatment with 1 μg/ml of DNAase and suspension in 10 mM CaCl2 and have enabled a three-dimensional analysis of the surface layer by negative staining.

Keywords

Negative Staining Surface Glycoprotein Shadow Casting Morphological Unit Halobacterium Halobium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • M. Kessel
    • 1
  • I. Wildhaber
    • 2
  • S. Cohen
    • 1
  • W. Baumeister
    • 2
  1. 1.Dept. of Membrane and Ultrastructure Research, Hadassah Medical SchoolThe Hebrew UniversityJerusalemIsrael
  2. 2.Max-Planck-lnstitut für BiochemieMartinsried bei MünchenGermany

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