Structure of the Surface Glycoprotein from Halobacterium volcanii as Revealed by Electron Microscopy
The cell surfaces of Halobacteria are seen by shadow casting to be composed of a hexagonal lattice of morphological units with a centre-to-centre spacing of approximately 15 nm. Preparations of virtually salt-free envelopes from Halobacterium volcanii, isolated from the Dead Sea, can be obtained by freezing and thawing the cells, treatment with 1 μg/ml of DNAase and suspension in 10 mM CaCl2 and have enabled a three-dimensional analysis of the surface layer by negative staining.
KeywordsDepression Hexagonal CaCl2 Uranyl Glycosaminoglycan
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- Kessel M, Wildhaber I, Cohen S, Baumeister W (1988) Three dimensional structure of the regular surface glycoprotein layer of Halobacterium volcanii from the Dead Sea. Proc Natl Acad Sci USA (submitted)Google Scholar