Computerized Digital Video Microdensitometry Applied to the Quantitation of Intracellular Macromolecules or Their Functional Groups
The use of specific histochemical stains to demonstrate features of cell metabolism or the state of cellular differentiation has provided much qualitative data about the biochemical status of cells and tissues. A quantitative estimate of a particular biochemical phenomenon may be obtained by using specific histochemical stains and microspectrophotometry (microdensitometry). In this way a measure of the variability in concentration of a substance in a cell population or within a tissue may be obtained, which could not be provided by biochemical assays which involve cellular disruption.
KeywordsReference Image Central Pixel Neutral Density Filter Output Processor Field Iris
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