Computerized Digital Video Microdensitometry Applied to the Quantitation of Intracellular Macromolecules or Their Functional Groups

  • D. J. Spargo
  • P. A. Riley

Abstract

The use of specific histochemical stains to demonstrate features of cell metabolism or the state of cellular differentiation has provided much qualitative data about the biochemical status of cells and tissues. A quantitative estimate of a particular biochemical phenomenon may be obtained by using specific histochemical stains and microspectrophotometry (microdensitometry). In this way a measure of the variability in concentration of a substance in a cell population or within a tissue may be obtained, which could not be provided by biochemical assays which involve cellular disruption.

Keywords

Flare Thiol Macromolecule Eicosanoid Disulphide 

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References

  1. Nöhammer G (1982) Quantitative microspectrophotometrical determination of protein thiols and disulphides with 2-2-dihydroxy-6-6-dinaphthyldisulphide (DDD). Histochemistry 75:219–250PubMedCrossRefGoogle Scholar
  2. Porro C, Fonda S, Baraldi P, Biral GP, Cavazzuti M (1984) Computer-assisted analyses of [14C]2-DC autoradiographs employing a general purpose image processing system. J Neurosci Methods 11:243–250PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • D. J. Spargo
    • 1
  • P. A. Riley
    • 1
  1. 1.Department of Chemical PathologyUniversity College and Middlesex School of MedicineLondonUK

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