Tf and Alpha-1-AT typing in bloodstains by Isoelectric Focusing

  • P. Cortivo
  • L. Caenazzo
  • C. Crestani
  • C. Scorretti
  • P. Benciolini
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

Conventional staining procedure for serum protein have employed the use of Coomassie Brilliant Blue R-250, however, with this staining is impossible to detect these proteins when they are in small quantities, like in forensic samples, even if a highly sensitive method as IEF are used.

Keywords

Sucrose Glycerol Electrophoresis Polyacrylamide Alanine 

References

  1. 1.
    Carracedo A., Conchiero L., Requena I., Lopez-Rivadulla M. A silver staining method for the detection of polymorphic proteins in minute bloodstains after isoelectric focusing For.Sci.Int. 23: 241–248 ( 1983 )Google Scholar
  2. 2.
    Dykes D.D., Miller S.A., Polesky H.F. Transferrin and Plasminogen phenotyping of aging bloodstains using isoelectric focusing and silver stain Electrophoresis 6: 90–93 ( 1985 )CrossRefGoogle Scholar
  3. 3.
    Henkeshoven J., Dernik R. Simplified method of silver staining of proteins in polyacrylamide gels and the mechanism of silver staining Electrophoresis 6: 103–112 ( 1985 )CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • P. Cortivo
    • 1
  • L. Caenazzo
    • 1
  • C. Crestani
    • 1
  • C. Scorretti
    • 1
  • P. Benciolini
    • 1
  1. 1.Istituto di Medicina Legale e delle Assicurazioni dell’Università di PadovaPadovaItaly

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