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Tf and Alpha-1-AT typing in bloodstains by Isoelectric Focusing

  • P. Cortivo
  • L. Caenazzo
  • C. Crestani
  • C. Scorretti
  • P. Benciolini
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

Conventional staining procedure for serum protein have employed the use of Coomassie Brilliant Blue R-250, however, with this staining is impossible to detect these proteins when they are in small quantities, like in forensic samples, even if a highly sensitive method as IEF are used.

Keywords

Glutamic Acid Brilliant Blue Silver Staining Ammonium Sulphate Coomassie Brilliant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Carracedo A., Conchiero L., Requena I., Lopez-Rivadulla M. A silver staining method for the detection of polymorphic proteins in minute bloodstains after isoelectric focusing For.Sci.Int. 23: 241–248 ( 1983 )Google Scholar
  2. 2.
    Dykes D.D., Miller S.A., Polesky H.F. Transferrin and Plasminogen phenotyping of aging bloodstains using isoelectric focusing and silver stain Electrophoresis 6: 90–93 ( 1985 )CrossRefGoogle Scholar
  3. 3.
    Henkeshoven J., Dernik R. Simplified method of silver staining of proteins in polyacrylamide gels and the mechanism of silver staining Electrophoresis 6: 103–112 ( 1985 )CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • P. Cortivo
    • 1
  • L. Caenazzo
    • 1
  • C. Crestani
    • 1
  • C. Scorretti
    • 1
  • P. Benciolini
    • 1
  1. 1.Istituto di Medicina Legale e delle Assicurazioni dell’Università di PadovaPadovaItaly

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