Identifying DNA RFLP’S in Routine Paternity Cases: Non-Isotopic Methods of Detection

  • D. Dykes
  • P. Watkins
  • D. Bowden
  • H. Polesky
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

An ever increasing number of polymorphic DNA probes have been reported in the literature. Thus it should be theoretically possible to select from a broad range of probes to be used in cases of disputed parentage. However, the lack of availability of many probes from the commercial sector, the general view that probes must be labeled with isotopes and various technical problems inherent with the techniques have hindered the widespread use of RFLP’s for this application.

Keywords

Agarose Electrophoresis Nylon Biotin 

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References

  1. Leary JJ, Brigati DJ, Ward DC. (1983) Rapid and sensitive colorimetric method for visualizing biotin labeled DNA or RNA immobilized on nitrocellulose: bio-dots. Proc Nat’l Acad Sci. USA. 80: 4045–4049.CrossRefGoogle Scholar
  2. Dykes D, Fondell J, Watkins P, Polesky H (1986) The use of biotinylated DNA probes for detecting single copy human restriction fragment length polymorphism by electrophoresis. Electrophoresis 7: 278–282.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • D. Dykes
    • 1
  • P. Watkins
    • 2
  • D. Bowden
    • 3
  • H. Polesky
    • 1
  1. 1.Memorial Blood Center of MinneapolisMinneapolisUSA
  2. 2.Integrated GeneticsFraminghamUSA
  3. 3.Collaborative ResearchBedfordUSA

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