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Genetic Polymorphism of Urinary Pepsinogen (PGA) Detected by Immunoblotting

  • T. Yasuda
  • K. Kishi
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

Pepsinogen is classified into two immunochemically distinct groups, PGA and PGC. PGA is detected in serum and urine as uropepsinogen, and while PGC is present in serum and seminal fluid. Genetic models of PGA have been proposed by several investigators (Samloff and Townes 1970; Taggart et al. 1979; Korsnes and Gedde-Dahl 1980; Frants et al. 1984), who have used all the proteolytic properties of acid-activated PGA for the detection of the isozymogens on agar or polyacrylamide gels. However, the complexities of these techniques have yielded a number of conflicting results, leading to various different genetic models.

Keywords

Genetic Model Human Urine Seminal Fluid Blotting Transfer Normal Human Urine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Frants FR, Pronk JC, Pals G, Defize J, Westerveld BD, Meuwissen SGM, Kreuning J, Eriksson AW (1984) Genetics of urinary pepsinogen: a new hypothesis. Hum Genet 65: 385–390PubMedCrossRefGoogle Scholar
  2. Kishi K, Iseki S (1983) Specific antibody to the young age-related glycoproteins (Ugl-Y) in normal human urine. Proc Japan Acad 59B: 240–242CrossRefGoogle Scholar
  3. Kishi K, Yasuda T, Iida R (1985) Glutamate-pyruvate transaminase (GPT) polymorphism detected in human urine by isoelectric focusing. Proc Japan Acad 61B: 490–493CrossRefGoogle Scholar
  4. Kishi K, Yasuda T (1987) Newly characterized genetic polymorphism of uropepsinogen group A (PGA) using both isoelectric focusing and immunoblotting. Hum Genet 75: 209–212PubMedCrossRefGoogle Scholar
  5. Korsnes L, Gedde-Dahl T Jr (1980) Genetics of pepsinogen I. Ann Hum Genet 43: 199–212PubMedCrossRefGoogle Scholar
  6. Minamiura N, Ito K, Kobayashi M, Kobayashi O, Yamamoto T (1984) Uropepsinogen in human urine: its protein nature, activation and enzymatic properties of activated enzyme. J Biochem 96: 1061–1069PubMedGoogle Scholar
  7. Samloff IM, Townes PL (1970) Pepsinogen: Genetic polymorphism in man. Science 168: 144–145PubMedCrossRefGoogle Scholar
  8. Taggart RT, Karn RC, Merritt AD, Yu PL, Conneally PM (1979) Urinary pepsinogen isozymogens: a highly polymorphic locus in man. Hum Genet 52: 227–238PubMedGoogle Scholar
  9. Weitkamp LR, Townes PL (1975) Genetics of the urinary pepsinogen isozymes. In: Markert CL (ed) Isozymes. vol 4: Genetics and evolution. Academic Press, New York, San Francisco, London, p 829–838Google Scholar
  10. Yasuda T, Ikehara Y, Sato W, Kishi K (1987) New detection method for uropepsinogen (PGA) using isoelectric focusing and immunoblotting techniques. Z Rechtsmed 98: 147–153PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • T. Yasuda
    • 1
  • K. Kishi
    • 1
  1. 1.Department of Legal MedicineFukui Medical SchoolMatsuoka-cho, FukuiJapan

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