α2-HS-Glycoprotein ( A2HS): Improved Phenotyping by Focusing in an Immobilized pH-Gradient. Allele Frequencies in a South German Population Sample and Linkage Data from a Large Pedigree

  • H. Lattke
  • C. Schönberger
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

The use of A2HS as an additional polymorphic marker in paternity testing and related forensic applications is not yet introduced in the routine expertises of all laboratories. One of several causes is the problem of antiserum supply, another the poor and batch-dependent resolution of the specific bands by separation with commercial carrier ampholytes according to previously described procedures (Weidinger, 1986; Mendner and Kühnl, 1986). Thus we developped an appropriate immobilized pH-gradient, which allows an improved and better reproducible separation.

Keywords

Glycerol Rubber Coomassie Persulfate TEMED 

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References

  1. Eiberg H, Mohr J, Nielson L S (1983) Human Gene Mapping 7. Cytogenetics and Cell Genetics 37 : 461Google Scholar
  2. Mendner T T, Kühnl P (1986) A2HS-Glykoprotein Polymorphismus. In: Brinkmann B and Henningsen K (eds.) Advances in Forensic Hemogenetics 1. Springer, Berlin Heidelberg New York, 167–174Google Scholar
  3. Weidinger S (1986) Isoelektrische Fokussierung und Printimmunfixation: Ein schnelles Verfahren zur Typisierung des A2-HS-Glykoproteins. In: Radola B J (ed) Elektrophorese Forum ’86. Bode, München, 156–162Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • H. Lattke
    • 1
  • C. Schönberger
    • 1
  1. 1.Abt. HumangenetikUniversität UlmUlmGermany

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