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Improved Diagnosis of Orosomucoid (ORM) Phenotypes by isoelectric focusing in immobilized pH gradients. Comparison with other phenotyping methods

  • A. Carracedo
  • M. D. Montiel
  • M. S. Rodriguez-Calvo
  • L. Concheiro
  • E. Huguet
  • M. Gené
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

Alpha-1-acid glycoprotein or orosomucoid (ORM) is a globulin characterized by a very high carbohydrate content (45%), a large number of syalyc residues and an extremely acidic isoelectric point (pI 2.7–3.5) (Schmid 1975).

Keywords

Silver Staining Legal Medicine Serum Group High Carbohydrate Content Codominant Inheritance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Carracedo A, Concheiro L, Requena I, Lopez-Rivadulla M (1983) A silver staining method for the detection of polymorphic proteins in minute bloodstains after IEF. For Sci Int 23: 241–248Google Scholar
  2. Carracedo A, Concheiro L, Estefania J, Suarez C (1985) Polymorphism of serum orosomucoid. Family and population studies in Galicia. Advances in Forensic Haemogenetics I. Springer-Verlag, Berlin, p 282Google Scholar
  3. Muñoz-Crego, MA (1987) Doctoral Thesis. Univ. Santiago de CompostelaGoogle Scholar
  4. Schmid K, Tokita K, Yooshizaki H (1965) The alpha-1-acid glycoprotein variants of normal caucasian and japanese individuals. J Clin Inves 44: 1394–1401CrossRefGoogle Scholar
  5. Schmid K (1975) Alpha-l-acid glycoprotein. In Putnam FW (ed) The plasma proteins, vol I. Academic Press, New York p 183Google Scholar
  6. Thymann M, Eiberg H (1985) Orosomucoid polymorphism: Determination by separator isoelectric focusing and demonstration of ORM F subtypes. In Brinkmann B, Henningsen H (eds.) Advances in Forensic Haemogenetics 1. Springer-Verlag, Berlin p 162Google Scholar
  7. Umetsu K, Ikeda N, Kashimura S, Suzuki T (1985) Orosomucoid (ORM) typing by print lectinofixation: a new technique for isoelectric focusing. Two common alleles in Japan. Hum Genet 71: 223–224PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • A. Carracedo
    • 1
  • M. D. Montiel
    • 1
  • M. S. Rodriguez-Calvo
    • 1
  • L. Concheiro
    • 1
  • E. Huguet
    • 2
  • M. Gené
    • 2
  1. 1.Dep. of Legal MedicineUniversity of Santiago de CompostelaSpain
  2. 2.Dep. of Legal MedicineUniversity of BarcelonaSpain

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