Advertisement

Subtyping of Factor B by Agarose Gel Electrophoresis

  • K. Suzuki
  • S. Ito
  • T. Harumoto
  • K. Fujita
  • Y. Matsuo
  • H. Matsumoto
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)

Abstract

Factor B (BF) polymorphism has been extended by the occurrence of subtypes in each of two common alleles, BF*F (Teng and Tan 1982; Geserick 1983; Abbal 1985; Nagai 1986; Nakamura 1987; Nishimukai 1987) and BF*S (David 1983; Weidinger 1984). Isoelectric focusing (IEF) has contributed to these studies, but there is a lack of correspondence among the subtypes postulated by each group or their frequencies. We present a BF F subtype using agarose gel electrophoresis (AGE) in Tris/glycine/Veronal buffer, further characterize them by zymosan treatment and by IEF in polyacrylamide gels (PAGIF) and also show a BF S subtype in the Hungarian population.

Keywords

Common Allele Osaka Medical College Veronal Buffer Hungarian Population Capillary Blotting 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abbal M, Thomsen M, Cambon-Thomsen A, Archambeau J, Calot M Fathallah D (1985). Hum Genet 69: 181–183PubMedCrossRefGoogle Scholar
  2. David V, Fauchet R, Phengsavath H, Guenet L, Le Gall JY (1983) Hum Genet 64: 189–190PubMedCrossRefGoogle Scholar
  3. Geserick G, Patzelt D, Schoder H, Nagai T (1983) Vox Sang 44: 178–182PubMedCrossRefGoogle Scholar
  4. Nagai T, Nagai T, Ikeda T (1986) Forensic Sci Int 30: 79–82PubMedCrossRefGoogle Scholar
  5. Nakamura S, Ooue O, Akiyama K, Abe K (1987) Hum Genet (in press)Google Scholar
  6. Nishimukai H, Tamaki Y (1987) Expl clin Immunogenet (to be submitted)Google Scholar
  7. O’Neill GJ, Yang SY, Dupont B (1978) Proc Natl Acad Sci USA 75: 5165–5169PubMedCrossRefGoogle Scholar
  8. Teisberg P (1970) Vox Sang 19: 47–56PubMedCrossRefGoogle Scholar
  9. Teng YS, Tan SG (1982) Hum Hered 32: 362–366PubMedCrossRefGoogle Scholar
  10. Weidinger S, Schwarzfisher F, Burgemeister R, Cleve H (1984) Hum Genet 68: 90–92PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • K. Suzuki
    • 1
  • S. Ito
    • 1
  • T. Harumoto
    • 1
  • K. Fujita
    • 1
  • Y. Matsuo
    • 2
  • H. Matsumoto
    • 1
  1. 1.Department of Legal MedicineOsaka Medical CollegeTakatsukiJapan
  2. 2.Department of Blood TransfusionOsaka Medical CollegeJapan

Personalised recommendations