Human Red Cell Acid Phosphatase (ACP1): Evidence for Differences in the Primary Structure of the two Isozymes Expressed by each Allele

  • J. Dissing
  • G. F. Sensabaugh
Conference paper
Part of the Advances in Forensic Haemogenetics book series (HAEMOGENETICS, volume 2)


One of the notable features of human red cell acid phosphatase (ACP1, E.C. is the generation of 2 isozymes (f and s) by each allele. These isozymes differ with respect to electrophoretic mobility (fig. 1) as well as to catalytic, stability and immunochemical properties (Harris 1980, Dissing 1987). The mechanism responsible for this pair wise production of isozymes has long been a puzzle and is of interest not only to the biochemist and geneticist but also to the forensic scientist.


Acid Phosphatase Reverse Phase HPLC Analysis Single Amino Acid Residue Immunochemical Property Dimensional Thin Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Dissing J (to be published) Immunochemical characterization of human red cell acid phosphatase isozymes. Biochem GenetGoogle Scholar
  2. Dissing J, Sensabaugh GF (to be published) Human red cell acid phosphatase (ACPI): Evidence for differences in the primary structure of the two isozymes encoded by the ACP1*B allele. Biochem GenetGoogle Scholar
  3. Dissing J, Dahl O, Svensmark O (1979) Phosphonic and arsonic acids as inhibitors of human red cell acid phosphatase and their use in affinity chromatography. Biochem Biophys Acta 569: 159–176PubMedGoogle Scholar
  4. Gracy RW (1977) Two dimensional thin layer methods. Methods Enzymol 47: 195–204PubMedCrossRefGoogle Scholar
  5. Harris H (1980) The Principles of Human Biochemical Genetics. Elsevier/North-Holland Biomedical Press, Amsterdam, New York, Oxford, pp 190–197.Google Scholar
  6. Leff SE, Rosenfeld MG, Evans RM (1986) Complex transcriptional units: diversity in gene expression by alternative RNA processing. Ann Rev Biochem 55: 1091–1117PubMedCrossRefGoogle Scholar
  7. Noguchi T, Inoue H, Tanaka T (1986) The M1- and M2- type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing. J Biol Chem 261: 13807–13812PubMedGoogle Scholar
  8. Rogers AP, Fisher RA, Putt W (1978) An Examination of the Age-Related Patterns of Decay of Acid Phosphatase (ACP1) in Human Red Cells from Individuals of Different Phenotypes. Biochem Genet 16: 727–737PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • J. Dissing
    • 1
  • G. F. Sensabaugh
    • 2
  1. 1.Institute of Forensic GeneticsUniversity of CopenhagenCopenhagenDenmark
  2. 2.Forensic Science Group, School of Public HealthUniversity of CaliforniaBerkeleyUSA

Personalised recommendations