Personal Identification by HLA Typing of Cultured Fibroblast Derived from Caderveric Tissues
It is well known that HLA typing of lymphocyte is extremely useful for personal identification and is often used for paternal testing in the field of legal medicine. Direct application of this method to personal identification of cadavers, however, is sometimes difficult because of relatively low viability of lymphocytes in a cadaver. Other method for HLA typing of a cadaver such as elution method reported by Kashiwade (1986) or absorption method (lymphocyte cytotoxicity inhibition test) reported by Yoshimura (1982) also have some difficulty that, in the case of a cadaver with completely unknown HLA type, these methods require a large amount of highly expensive HLA antibodies, consumes tremendous time and, thus, is not applicable for practical purposes. In an effort to find easy and general application of HLA typing of cadaver tissues, we investigated the possibility of carrying out the test by using conventional typing tray. In this report, we will show that HLA typing of a cadaver can be successfully made by using cultured fibroblast and slightly modifying the conventional NIH method for HLA typing of lymphocytes (Bodmer, 1978).
KeywordsPersonal Identification Living Body Legal Medicine Paternal Testing Elution Method
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