Rapid, Even Distribution of Pollen Populations on Semi-Solid Media Using a Modified Replica Plate Technique
Pollen populations are proving to be very useful tools for a wide variety of studies, including the measurement of biotic and abiotic stresses. A majority of these studies require fresh pollen which readily germinate on appropriate selected artificial media. A second requirement is the even distribution of single pollen grains on the medium so that numbers of pollen grains, rate of germination, and tube lengths are easily measured. Brush and other gross dusting techniques produce clumpy, uneven population distribution on semi solid media. This poster describes a simple technique which produces even population distribution.