Abstract
The present study is partly a consequence of our interspecific cross research programme in Lilium spp. (Van Tuyl et al., 1987). Van Roggen et al. (1988) demonstrated that sperm cells generally do not reach the embryosac following the cut-style pollination method which is necessary for interspecific lily crosses. As a first step to study the fate of sperm cells in a non-specific embryo sac, we developed a system for artificial (intraspecific) sperm cell injection into the embryo sac. For this, Torenia fournieri was used as a model plant, since the embryo sac emerges from the integuments in this species (Van der Pluijm, 1964; Wilms and Keijzer, 1985).
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© 1988 Springer-Verlag Berlin Heidelberg
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Keijzer, C.J., Reinders, M.C., Leferink-ten Klooster, H.B. (1988). A Micromanipulation Method for Artificial Fertilization in Torenia . In: Cresti, M., Gori, P., Pacini, E. (eds) Sexual Reproduction in Higher Plants. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73271-3_19
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DOI: https://doi.org/10.1007/978-3-642-73271-3_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73273-7
Online ISBN: 978-3-642-73271-3
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