Advertisement

Sequence Determinants of Protein Sorting Into and Across Membranes

  • Gunnar von Heijne
Conference paper
Part of the NATO ASI Series book series (volume 16)

Abstract

My intentions with this contribution are twofold: to describe briefly some methods of computer-aided sequence analysis in molecular biology, and to review the most important results obtained through such theoretical studies in the related fields of protein sorting and membrane protein biogenesis.

Keywords

Signal Peptide Mitochondrial Target Sequence Secretory Signal Peptide Eukaryotic Sequence Hydrophobic Moment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Blobel, G. (1980) Intracellular Protein Topogenesis. Proc.Natl.Acad.Sci.USA 77, 1496–1500.PubMedCrossRefGoogle Scholar
  2. Cornette, J.L., Cease, K.B., Margalit, H., Spouge, J.L., Berzofsky, J.A., & DeLisi, C. (1987) Hydrophobicity Scales and Computational Techniques for Detecting Amphipathic Structures in Proteins. J.Mol.Biol. 195, 659–685.PubMedCrossRefGoogle Scholar
  3. Dalbey, R.E., Kuhn, A., & Wickner, W. (1987) J.Biol.Chem. in press.Google Scholar
  4. Dalbey, R.E., & Wickner, W. (1987) Leader Peptidase of Escherichia coli: Critical Role of a Small Domain in Membrane Assembly. Science 235, 783–787.PubMedCrossRefGoogle Scholar
  5. Engelman, D.M., & Steitz, T.A. (1981) The Spontaneous Insertion of Proteins Into and Across Membranes: The Helical Hairpin Hypothesis. Cell 23, 411–422.PubMedCrossRefGoogle Scholar
  6. Eisenberg, D., Schwarz, E., Komaromy, M., & Wall, R. (1984) Analysis of Membrane and Surface Protein Sequences with the Hydrophobic Moment Plot. J.Mol.Biol. 179, 125–142.PubMedCrossRefGoogle Scholar
  7. Finer-Moore, J., & Stroud, R.M. (1984) Amphipathic Analysis and Possible Formation of the Ion Channel in an Acetylcholine Receptor. Proc. Natl. Acad. Sci. USA 81, 155–159.PubMedCrossRefGoogle Scholar
  8. Moore, K., & Miura, S. (1987) J.Biol.Chem. in pressGoogle Scholar
  9. Perlman, D., & Halvorson, H.O. (1983) A Putative Signal Peptidase Recognition Site and Sequence in Eukaryotic and Prokaryotic Signal Peeptides. J. Mol. Biol. 167, 391–409.PubMedCrossRefGoogle Scholar
  10. Rapoport, T. (1986) Protein Translocation Across and Integration Into Membranes CRC Crit. Rev. Biochem. 20, 73–137.PubMedCrossRefGoogle Scholar
  11. Vogel, H., & Jähnig, F. (1986) Models for the Structure of Outer-Membrane Proteins of Escherichia coli Derived from Raman Spectroscopy and Prediction Methods. J.Mol.Biol. 190, 191–199.PubMedCrossRefGoogle Scholar
  12. von Heijne, G. (1983) Patterns of amino Acids near Signal-Sequence Cleavage Sites. Eur.J.Biochem. 133, 17–21.CrossRefGoogle Scholar
  13. von Heijne, G. (1984) Analysis of the Distribution of Charged Residues in the N-terminal Region of Signal Sequences: Implications for Protein Export in Prokaryotic and Eukaryotic Cells. EMBO J. 3, 2315–2318.Google Scholar
  14. von Heijne, G. (1985) Signal Sequences: The Limits of Variation. J.Mol.Biol. 184, 99–105.CrossRefGoogle Scholar
  15. von Heijne, G. (1986a) The Distribution of Positively Charged Residues in Bacterial Inner Membrane Proteins Correlates with the Trans-Membrane Topology. EMBO J. 5, 3021–3027.PubMedGoogle Scholar
  16. von Heijne, G (1986b) A New Method for Predicting Signal Sequence Cleavage Sites. Nucl.Acids Res. 14, 4683–4690.CrossRefGoogle Scholar
  17. von Heijne, G. (1986c) Mitochondrial Targeting Sequences May Form Amphiphilic Helices. EMBO J. 5, 1335–1342.Google Scholar
  18. von Heijne, G. (1987) Sequence Analysis in Molecular Biology: Treasure Trove or Trivial Pursuit? Academic Press, New York.Google Scholar
  19. von Heijne, G., Wickner, W., & Dalbey, R. (1987) The Cytoplasmic Domain of Escherichia coli Leader Peptidase is a Translocation Poison Sequence. Submitted.Google Scholar
  20. Wickner, W., & Lodish, H. (1985) Multiple Mechanisms of Protein Insertion Into and Across Membranes. Science 230, 400–407.PubMedCrossRefGoogle Scholar
  21. Wolfe, P.B., Wickner, W., & Goodman, J. (1983) Sequence of the Leader Peptidase Gene of Escherichia coli and the Orientation of Leader Peptidase in the Bacterial Envelope. J.Biol.Chem. 258, 12073–12080.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Gunnar von Heijne
    • 1
  1. 1.Research Group for Theoretical Biophysics, Department of Theoretical PhysicsRoyal Institute of TechnologyStockholmSweden

Personalised recommendations