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A Novel Toxin Produced by Pseudomonas Corrugata, the Causal Agent of Tomato Pith Necrosis: Determination of its Role in Virulence and the Genetics of Production

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Phytotoxins and Plant Pathogenesis

Part of the book series: NATO ASI Series ((ASIH,volume 27))

Abstract

Pseudomonas corrugata, the causal agent of tomato pith necrosis, was first reported in greenhouse-grown tomatoes in 1978 (Scarlett et al., 1978) and in field-grown tomatoes in southern California in 1983 (Lai et al., 1983). The characteristic symptom of the disease is a black necrotic pith that becomes hollow and causes the stem to collapse in severely infected plants. Inoculation of bacterial cells into leaves of tomato plants results in formation of necrotic lesions in one to two days. During initial pathogenicity tests, three- to four-week-old tomato plants in the greenhouse were killed when inoculated by stem injection and incubated under conditions of high humidity and/or high nitrogen. Total collapse of plants occurred within 24 to 48 h with inoculum as low as 104 colony forming units (cfu)/ml. It was also observed that cell-free culture fluids from four-day-old cultures of P. corrugata produced necrotic lesions similar to those produced when bacterial cells were infiltrated into tomato leaves. These results suggested that a toxin may be involved in pathogenicity and/or virulence.

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© 1989 Springer-Verlag Berlin Heidelberg

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Chun, W., Leary, J.V. (1989). A Novel Toxin Produced by Pseudomonas Corrugata, the Causal Agent of Tomato Pith Necrosis: Determination of its Role in Virulence and the Genetics of Production. In: Graniti, A., Durbin, R.D., Ballio, A. (eds) Phytotoxins and Plant Pathogenesis. NATO ASI Series, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73178-5_9

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  • DOI: https://doi.org/10.1007/978-3-642-73178-5_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73180-8

  • Online ISBN: 978-3-642-73178-5

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