Effect of Nerve Stimulation, K+ Saline and Hypertonic Saline on Classes of Quanta, Quantal Content and Synaptic Vesicle Size Distribution of Torpedo Electric Organ
The Torpedo electric organ is an extremely useful preparation for the study of cholinergic mechanisms because of its easy accessibility to biochemical, morphological and physiological methods. A kilogram of electric organ can be obtained from one animal of which 10% are synaptic terminals. At least 3 classes of synaptic vesicles have been described based on density (Stadler and Kiene, 1987) and 2 classes based on diameter (Zimmermann and Whittaker, 1974, 1977). Its unique structure of vertical columns of stacked electrocytes enables one to uniformly field stimulate the tissue and obtain reliable quantitative end-plate potential.
KeywordsSynaptic Vesicle Hypertonic Saline Sodium Cacodylate MEPP Amplitude Electric Organ
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