Abstract
Synapses are the structural elements of the neuronal network through which cellular communication occurs. A prerequisite for understanding synaptic information transfer is the identification of the various components of the synaptic machinery. Recently, different molecular genetic techniques have been developed for the isolation of neuron-specific gene products, such as differential screening, gene transfer, deletion mutant analysis, and screening of expression libraries with monoclonal antibodies or selective ligands. Here, we describe two cloning approaches which have been successfully used in our laboratory. The first illustrates how neural gene products can be isolated and identified by selectively cloning mRNAs which appear in the avian optic lobe during the major period of synaptogenesis.
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References
Aprison, M.H. and E.C. Daly. 1978. Biochemical aspects of transmission at inhibitory synapses: the role of glycine. Advances in Neurochemistry 3: 203–294.
Becker, C.-M., I. Hermans-Borgmeyer, B. Schmitt and H. Betz. 1986. The glycine receptor deficiency of the mutant mouse spastic: evidence for normal glycine receptor structure and localization. J. Neurosci. 6: 1358–1364.
Benavides, J., J. Lopez-Lahoya, F. Valdivieso and M. Ugarte. 1981. Postnatal development of synaptic glycine receptors in normal and hyperglycinemic rats. J. Neurosci. 37: 315–320.
Betz, H. 1987. Biology and structure of the mammalian glycine receptor. Trends in Neurosci. 10: 113–117.
Cleveland, D.W., M.A. Lopata, R.J. MacDonald, N.J. Cowan, W.J. Rutter and M.W. Kirschner. 1980. Number and evolutionary conservation of α- and ß-Tubulin and cytoplasmic ß- and g-Actin genes using specific cloned cDNA probes. Cell 20: 95–105.
Davis, M.M., D.I. Cohen, E.A. Nielsen, M. Steinmetz, W.E. Paul and L. Hood. 1984. Cell-type specific cDNA probes and the murine I region: The localization and orientation of Aα. Proc. Natl. Acad. Sci. USA 81: 2194–2198.
Graham, D., F. Pfeiffer and H. Betz. 1983. Photoaffinity-labelling of the glycine receptor of rat spinal cord. Eur. J. Biochem. 131: 519–525.
Graham, D., F. Pfeiffer, R. Simler and H. Betz. 1985. Purification of the glycine receptor of pig spinal cord. Biochemistry 24: 990–994.
Grenningloh, G., A. Rienitz, B. Schmitt, C. Methfessel, M. Zensen, K. Beyreuther, E.D. Gundelfinger and H. Betz. 1987. The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors. Nature 328: 215–220
Gubler, K. and B.J. Hoffmann. 1983. A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269.
Hunt, P.H. and N. Brecha. 1984. The avian tectum: A synthesis of morphology and biochemistry. In Comparative neurology of the optic tectum (ed. H. Venegas ) pp. 619–648. Plenum, New York and London.
Julien, J.P. and W.E. Mushynski. 1982. Multiple phosphorylation sites in mammalian neurofilament polypeptides. J. Biol. Chem. 257: 10467–10470.
Julien, J.-P., D. Meyer, D. Flavell, J. Hurst, and F. Grosveld. 1986. Cloning and developmental expression of the murine neurofilament gene family. Mol. Brain Res. 1: 243–250.
Myers, M.W., R.A. Lazzarini, V. M.-Y. Lee, W.W. Schlaepfer and D.L. Nelson. 1987. The human mid-size neurofilament subunit: a repeated protein sequence and the relatioship of its gene to the intermediate filament gene family. EMBO J. 6: 1617–1626.
Pfeiffer, F., D. Graham and H. Betz. 1982. Purification by affinity chromatography of the glycine receptor of rat spinal cord. J. Biol. Chem. 257: 9389–9393.
Schmitt, B., P. Knaus, C.-M. Becker and H. Betz. 1987. The Mr 93000 polypeptide of the postsynaptic glycine receptor complex is a peripheral membrane protein. Biochemistry 26: 805–811.
Schofield, P.R., M.G. Darlison, N. Fujita, D.R. Burt, F.A. Stephenson, H. Rodriguez, L.M. Rhee, J. Ramachandran, V. Reale, T.A. Glencorse, P.H. Seeburg and E.A. Barnard. 1987. Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family. Nature 328: 221–227.
Shivers, B., B.S. Schachter and D.W. Pfaff. 1986. In situ hybridization for the study of gene expression in the brain. Methods Enzymol. 124: 497–510.
Sumikawa, K., I. Parker and R. Miledi. 1984. Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels. Proc. Natl. Acad. Scd. 81: 7994–7998.
Weber, K., G. Shaw, M. Osborn, E. Debus, and N. Geisler. 1983. Neurofilaments, a subclass of intermediate filaments: structure and expression. Cold Spring Harbour Symp. Quant. Biol. 46: 717–729.
Zopf, D., I. Hermanns-Borgmeyer, E.D. Gundelfinger and H. Betz. 1987. Identification of gene products expressed in the developing chick visual system: characerization of a middle- molecular-weight neurofilament cDNA. Genes and Development 1: 699–708.
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© 1988 Springer-Verlag Berlin Heidelberg
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Zopf, D. et al. (1988). Molecular Biology Approaches to the Function and Development of CNS Synapses.. In: Zimmermann, H. (eds) Cellular and Molecular Basis of Synaptic Transmission. NATO ASI Series, vol 21. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73172-3_34
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DOI: https://doi.org/10.1007/978-3-642-73172-3_34
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