Synaptophysin: A Major Cell Type-Specific Vesicle Protein of Neuroendocrine Cells

  • Bertram Wiedenmann
  • Werner W. Franke
Conference paper
Part of the NATO ASI Series book series (volume 21)


In recent years, three integral membrane proteins, which appear to be specifically associated with small (40–80 nm) vesicles with electron microscopically translucent contents have been detected in various neuroendocrine (NE) cells, primarily by the use of monoclonal antibodies. On SDS-polyacryl amide gel electrophoresis (PAGE) of proteins from brain tissue homogenates, these molecules appear with relative mobilities corresponding to Mr 95,000 (protein termed SV2; Buckley and Kelly, 1985), Mr 65,000 (Matthew et al., 1981) and Mr 38,000 (termed synaptophysin; Jahn et al., 1985; Wiedenmann and Franke, 1985). Particular progress has recently been made in our understanding of the molecular and cellular topology and distribution of synaptophysin.


Synaptic Vesicle Neuroendocrine Cell Coated Vesicle Synaptic Vesicle Protein Presynaptic Vesicle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Buckley, K., Kelly, R.B. (1985): Identification of a trans-membrane glycoprotein specific for secretory vesicles of neural and endocrine cells. J. Cell Biol. 100: 1284–1294.PubMedCrossRefGoogle Scholar
  2. 2.
    Buckley, K.M., Floor, E., Kelly, R.B. (1987): Cloning and sequence analysis of cDNA encoding p38, a major synaptic vesicle protein. J. Cell Biol. 105: 2447–2456.PubMedCrossRefGoogle Scholar
  3. 3.
    Chejfec, G., Falkmer, S., Grimelius, L., Jacobsson, B., Rodensjö, M., Wiedenmann, B., Franke, W.W., Gould, V.E. (1987): Synaptophysin, a new marker for pancreatic neuroendo crine tumors. Am. J. Surg. Pathol. 11: 241–247.PubMedCrossRefGoogle Scholar
  4. 4.
    Franke, W.W., Grund, C., Achtstätter, T. (1986): Coexpression of cytokeratins and neurofilament proteins in a permanent cell line: cultured rat PC12 cells combine neuronal and epithelial features. J. Cell Biol. 103: 1933–1943.PubMedCrossRefGoogle Scholar
  5. 5.
    Gould, V.E., Wiedenmann, B., Lee, I., Schwechheimer, K., Dockhorn-Dworniczak, B., Radosevich, J.A., Moll, R., Franke, W.W. (1987): Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry. Am. J. Pathol. 126: 243–257.PubMedGoogle Scholar
  6. 6.
    Hofmann, W.J., Waldherr, R., Eberlein-Gonska, M., Wiedenmann, B., Otto, H.F.: Imnunohistochemical examination of carcinoids of the thymus and thymomas with neuroendocrine markers, in preparation.Google Scholar
  7. 7.
    Jahn, B., Schiebler, W., Ouimet, C., Greengard, P. (1 985): A 38 000 dalton membrane protein (p38) present in synaptic vesicles. Proc. Natl. Acad. Sci. USA 82: 4137–4141.PubMedCrossRefGoogle Scholar
  8. 8.
    Kayser, W., Wiedenmann, B. (1988): Neuroendocrine markers in bronchial carcinomas. A comparison of synaptophysin neuron- specific enolase and bombesin. Pathol. Res. Pract., in press.Google Scholar
  9. 9.
    Knaus, P., Betz, H., Rehm, H. (1986): Expression of synaptophysin during postnatal development of the mouse brain. J. Neurochem. 47: 1302–1304.PubMedCrossRefGoogle Scholar
  10. 10.
    Lee, I., Gould, V.E., Moll, R., Wiedenmann, B., Franke, W.W. (1987): Synaptophysin expressed in the broncho-pul monary tract: Neuroendocrine cells, neuroepithelial bodies and neuroendocrine neoplasms. Differentiation 34: 115–125.Google Scholar
  11. 11.
    Leube, R.E., Kaiser, P., Sciter, A., Zimbelmann, R., Franke, W.W., Rehm, H., Knaus, P., Prior, P., Betz, H., Reinke, H., Beyreuther, K., Wiedenmann, B. (1987): Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA. EMB0 J. 6: 3261–3268.Google Scholar
  12. 12.
    Navone, F., Jahn, R., Di Gioia, G., Stukenbrok, G., Greengard, P., De Camilli, P. (1986): Protein p38: an integral membrane protein specific for small vesicles of neurons and neuroendocrine cells. J. Cell Biol. 103: 2511–2527.PubMedCrossRefGoogle Scholar
  13. 13.
    Pfeffer, S.R., Kelly, R.B. (1985): The subpopulation of brain coated vesicles that carries synaptic vesicle proteins contains two unique polypeptides. Cell 40: 949–957.PubMedCrossRefGoogle Scholar
  14. 14.
    Rehm, H., Wiedenmann, B., Betz, H. (1986): Molecular characterization of synaptophysin, a major calcium-binding protein of the synaptic vesicle membrane. EMBO J. 5: 535–541.PubMedGoogle Scholar
  15. 15.
    Schwechheimer, K., Wiedenmann, B., Lee, I., Franke, W.W. (1987): Synaptophysin: a reliable marker for medullo-blastomas. Virchows Arch. A 411: 53–59.CrossRefGoogle Scholar
  16. 16.
    Südhof, T.C., Lottspeich, F., Greengard, P., Mehl, E., Jahn, R. (1987): A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions. Science 238: 1142–1144.PubMedCrossRefGoogle Scholar
  17. 17.
    Ti xier-Vi dal, A., Faivre-Bauman, A., Picart, R., Wiedenmann, B. (1988): Immunoelectron microscopic localization of synaptophysin in a Golgi subcompartment of developing hypothalamic neurons. Neuroscience, in press.Google Scholar
  18. 18.
    Wiedenmann, B., Franke, U.U. (1985): Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles. Cell 41: 1017–1028.PubMedCrossRefGoogle Scholar
  19. 19.
    Wiedenmann, B., Lawley, K., Grund, C., Branton, D. (1985): Solubilization of proteins from bovine brain coated vesicles by protein perturbants and Triton X–100. J. Cell Biol. 101: 12–18.PubMedCrossRefGoogle Scholar
  20. 20.
    Wiedenmann, B., Franke, W.W., Kuhn, C., Moll, R., Gould, V.E. (1986): Synaptophysin: a marker protein for neuroendocrine cells and neoplasms. Proc. Natl. Acad. Sci. USA 83: 3500–3504.PubMedCrossRefGoogle Scholar
  21. 21.
    Wiedenmann, B., Kuhn, C., Schwechheimer, K., Waldherr, R., Raue, F., Brandeis, W.E., Kommerei 1, B., Franke, W.W. (1987): Synaptophysin identified in metastases of neuroendocrine tumors by immunocytochemistry and immunoblotting. Am. J. Clin. Pathol. 87: 560–569.Google Scholar
  22. 22.
    Wiedenmann, B., Rehm, H., Franke, W.W. (1987): Synaptophysin, an integral membrane protein of vesicles present in normal and neoplastic neuroendocrine cells. Ann. N. Y. Acad. Sci. 463: 501–503.Google Scholar
  23. 23.
    Wiedemann, B., Waldherr, R., Rosa, P., Huttner, W.: Gastro-enteropancreatic neuroendocrine cells and tumors, identified by proteins specifically associated to dense and clear vesicles, using synaptophysin, chromogranin A and B, and secretogranin II. Gastroenterology, submitted.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Bertram Wiedenmann
    • 1
    • 2
  • Werner W. Franke
    • 1
    • 2
  1. 1.Med. Univ. Klinik, Department of MedicineUniversity of HeidelbergHeidelbergGermany
  2. 2.Institute of Cell and Tumor BiologyGerman Cancer Research CenterHeidelbergGermany

Personalised recommendations