Reduced Growth Rate and Changes in Cell Wall Proteins of Plant Cells Adapted to NaCl
Plant cells which are exposed to sufficient osmotic stress will lose turgor and stop growing. However, within limits plant cells will osmotically adjust to such stress and restore turgor but growth remains inhibited. We have hypothesized that osmotically adapted cells have restricted growth rates because of alterations in cell wall metabolism. We demonstrate here that the amounts of hydroxyproline rich glycoproteins are greatly reduced in adapted cells and, hence, cannot be involved in restricted growth. Adapted cells do have an altered pattern of ionically-bound cell wall proteins and proteins released outside of the cell. Any role of these protein changes in restricting cell growth remains to be proven. Plants regenerated from adapted cells exhibit a genetically stable reduction in leaf cell enlargement rate. Alterations in cell wall properties of these leaf cells remain to be determined.
KeywordsHydrolysis Filtration Tyrosine Glycine Polysaccharide
Unable to display preview. Download preview PDF.
- Bressan RA, Singh NK, Handa AK, Kononowicz A, Hasegawa PM (1985) Stable and unstable tolerance to NaCl in cultured tobacco cells. In: Freeling M (ed) UCLA Symposium on Plant Genetics. AR Liss, New York, pp 755–769Google Scholar
- Bressan RA, Singh NK, Handa AK, Mount R, Clithero J, Hasegawa PM (1987) Stability of altered genetic expression in cultured plant cells adapted to salt. In: Monti L, Porceddu E (eds). Drought Resistance in Plants: Physiological and Genetic Aspects. Comm Europ Commun, Luxembourg, pp 41–58Google Scholar
- Briggs LJ, Shantz HL (1914) Relative water requirement of plants. J Agricultural Res 3(1):1–64Google Scholar
- Hasegawa PM, Bressan RA, Handa S, Handa AK (1984) Cellular mechanisms of tolerance to water stress. HortScience 19(3): 371–376Google Scholar
- Hasegawa PM, Bressan RA, Handa AK (1986) Cellular mechanisms of salinity tolerance. HortScience 21(6)1317–1324Google Scholar
- Iraki NM, Bressan RA, Hasegawa PM, Carpita NC (1988a) Alteration of the physical and chemical structure of the primary cell wall of growth limited plant cells adapted to osmotic stress. Plant Physiol In PressGoogle Scholar
- Iraki NM, Singh NK, Bressan RA, Carpita NC (1988b) Cell walls of tobacco cells and changes in composition associated with reduced growth upon adaptation to water and salt stress. Plant Physiol In PressGoogle Scholar
- Iraki NM, Bressan RA, Carpita NC (1988c) Extracellular polysaccharides and proteins of tobacco cell cultures and changes in composition with growth-limiting adaptation to water and saline stress. Plant Physiol In PressGoogle Scholar
- Nevins DJ, Hatfield R, Hoson T, Inouhe M (1987) Inhibition of elongation by antibodies specific for wall proteins. In: Cosgrove DJ, Knieval DP (eds) Physiology of Cell Expansion During Plant Growth. Amer Soc Plant Physiol, Rockville, MD In PressGoogle Scholar
- Song C-S, Yu J-H, Bai DH, Hester PY, Kim K-H (1985) Antibodies to the α-subunit of insulin receptor from eggs of immunized hens. J Immunology 135:3354–3359Google Scholar
- Wilson LG, Fry JC (1986) Extension — a major cell wall glycoprotein. Plant Cell Environment 9:239–260Google Scholar