In vivo Fluorescence from Mouse Skin: Spectral Evidence for the Presence of Chlorophyll Derivatives

  • P. E. Paterson
  • G. Weagle
  • R. Pottier
  • J. Kennedy
Conference paper
Part of the NATO ASI Series book series (volume 15)

Abstract

The technique of fluorescence spectroscopy is a convenient and rapid physical method for the detection and monitoring of chemicals in vivo. It has recently been shown that such a technique can easily monitor the biosynthesis and subsequent clearance of protoporphyrin IX (PP) in the skin of non-anesthetized mice that had been given various doses of the porphyrin precursor 5-aminolevulinic acid (ALA) (1). It was also reported in that study that the background fluorescence spectrum of live mice contained a significant band centered near 674 nm, but no assignment was made for this band. We report here evidence that supports the assignment of this background fluorescence as arising from partially digested mouse food.

Keywords

Quartz HPLC Chlorophyll Mercury Photosynthesis 

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References

  1. 1.
    Pottier, R.H., Chow, Y.F.A., LaPlante, J.P., Truscott, T.G., Kennedy, J.C. and Beiner, L.A. (1986), Non-invasive Technique for Obtaining Flourescence Excitation and Emission Spectra in vivo, Photochem. Photobiol. 44 (5): 679–687.CrossRefGoogle Scholar
  2. 2.
    Brasseur, N., Hasrat, A., Langlois, R., Wagner, J.R., Rousseau, J. and van Lier, J.E. (1987) Biological activities of phthalocyanines-V. Photodynamic therapy of EMT-6 mammary tumors in mice with sulfonated phthalocyanines. Photochem. Photobiol. 45, 581–586.Google Scholar
  3. 3.
    Jones, O.T.G. (1973) Chlorophyll, in Phytochemistry: The Process and Products of Photosynthesis, Vol. 1, L.P. Miller, ed., Van Nostrand Reinhold Reinhold Company, Toronto.Google Scholar
  4. 4.
    Hill, R. (1963) Chlorophyll, in Comprehensive Biochemistry: Volume 9 Pyrrole Pigments, Isoprenoid Compounds and Phenolic Plant Constituents, M. Florkin and E.H. Stotz, eds., Elesvier Publishing Company, New York.Google Scholar
  5. 5.
    Goedheer, J.C. (1966) Visible Absorption and Fluorescence of Chlorophyll and Its Aggregates in Solution, in The Chlorophylls, L.P. Vernon and G.R. Seely, eds., Academic Press, New York.Google Scholar
  6. 6.
    Tapper, B.A. Lohrey, E., Howe, E.L. and Allison, R.M. (1975) Photosensitivity from Chlorophyll-Derived Pigments, J.Sci.Fd Agric. 26: 277–284.CrossRefGoogle Scholar
  7. 7.
    Brown, S.R. (1968) Absorption Coefficients of Chlorophyll Derivatives, J. Fish. Res. Bd Can. 25 (3): 523–540.CrossRefGoogle Scholar
  8. 8.
    Daley, R.J., Gray, C.B.J, and Brown, S.R. (1973) Reversed Phase Thin-Layer Chromatography of Chlorophyll Derivatives, J. Chromat. 76: 175–183.CrossRefGoogle Scholar
  9. 9.
    Roder, B. (1986) Pheophorbide a - A New Photosensitizer for the Photodynamic Therapy of Tumours, Studia Biophysica 114: 183–186.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • P. E. Paterson
    • 1
  • G. Weagle
    • 1
  • R. Pottier
    • 1
  • J. Kennedy
    • 1
    • 2
  1. 1.Department of Chemistry and Chemical EngineeringRoyal Military College of CanadaKingstonCanada
  2. 2.Dept. of OncologyQueen’s UniversityKingstonCanada

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