In vivo Fluorescence from Mouse Skin: Spectral Evidence for the Presence of Chlorophyll Derivatives
The technique of fluorescence spectroscopy is a convenient and rapid physical method for the detection and monitoring of chemicals in vivo. It has recently been shown that such a technique can easily monitor the biosynthesis and subsequent clearance of protoporphyrin IX (PP) in the skin of non-anesthetized mice that had been given various doses of the porphyrin precursor 5-aminolevulinic acid (ALA) (1). It was also reported in that study that the background fluorescence spectrum of live mice contained a significant band centered near 674 nm, but no assignment was made for this band. We report here evidence that supports the assignment of this background fluorescence as arising from partially digested mouse food.
KeywordsQuartz HPLC Chlorophyll Mercury Photosynthesis
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