Abstract
31P-NMR techniques allow the continuous, non-destructive measurements of phosphorous compounds in intact cells. Immediate effects of photodynamic treatment and photosensitization processes can be mapped [2,6]. 31P-NMR also gives information about changes in the pH gradient across membranes and indirectly about ion pumping mechanisms controlling proton fluxes [3]. We have studied the bacterium Propionibacterium acnes, (P. acnes) which produces large amounts of endogenous porphyrins taking part in photosensitization [5]. Externally applied HpD is effective in increasing the photosensitivity [4]. In the present short report we would like to present some new experimental results where 31P-NMR has been used to analyse photodynamic reactions of red light and HpD treatment.
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References
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© 1988 Springer- Verlag Berlin Heidelberg
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Kjeldstad, B., Johnsson, A., Krane, J. (1988). Photoinactivation of cells studied by 31P-NMR. In: Moreno, G., Pottier, R.H., Truscott, T.G. (eds) Photosensitisation. NATO ASI Series, vol 15. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73151-8_29
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DOI: https://doi.org/10.1007/978-3-642-73151-8_29
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