Abstract
Oxidative phosphorylation takes place in mitochondria and furnishes mammalian cells with the majority of ATP necessary for metabolism. Moreover mitochondria play an essential role in the regulation of free Ca2+, an ion which is considered to be a second messenger. Consequently damage to these organelles can perturb functioning tremendously when cells are irradiated in the presence of various photosensitizers. Lipophilic molecules able to give singlet oxygen with a large quantum yield such as many porphyrins are well suited to sensitizing mitochondria. In vivo, porphyrins photosensitization has a noxious effect in cutaneous porphyrias which are porphyria cutanea tarda with accumulation of uro- and heptaporphyrin and erythropoietic protoporphyria with accumulation of protoporphyrin. The action of hematoporphyrin (Hp) and hematoporphyrin derivative (HpD) used in photo-chemotherapy has also been extensively studied. In both cases, the mechanism of the subcellular damage remains rather obscure and the first part of this paper deals with a discussion of the importance of mitochondria as subcellular targets. In the second part, the in vitro effects of irradiation on mitochondrial enzymes and mitochondrial functions are reported in oxic and anoxic conditions.
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References
Atlante A, Moreno G, Passarella S, Salet C (1986) Hematoporphyrin derivative (Photofrin II) photosensitization of isolated mitochondria: impairment of anion translocation. Biochem Biophys Res Comm 141: 584–590
Bazin M, Santus R (1986) Selective type I photooxidations in mixtures of porphyrins and electrophilic nitroimidazoles. Photochem Photobiol 43: 235–242
Berns MW, Dahlman A, Johnson FM, Burns R, Serling D, Guiltinan M, Siemens A, Walter R, Wright W, Hammer-Wilson M, Wile A (1982) In vitro cellular effects of hematoporphyrin derivative. Cancer Res 42: 2325–2329.
Coppola A, Viggiani E, Salzarulo L, Rasile G (1980) Ultrastructural changes in lymphoma cells treated with hematoporphyrin and light. Am J Pathol 99: 175–192.
Cozzani I, Jori G, Reddi E, Fortunato A, Granati B, Felice M, Tomio L, Zorat P (1981) Distribution of endogenous and injected porphyrins at the subcellular level in rat hepatocytes and in ascites hepatoma. Chem Biol Interactions 37: 67–75
Gibson SL, Hilf R (1985) Interdependence of fluence, drug dose and oxygen on hematoporphyrin derivative induced photosensitization of tumor mitochondria. Photochem Photobiol 42: 367–373.
Grossweiner LI, Goyal GC (1983) Photosensitized lysis of liposomes by hematoporphyrin derivative. Photochem Photobiol 37: 529–532.
Hilf R, Murant RS, Narayanan U, Gibson SL (1986) Relationship of mitochondrial function and cellular adenosine triphosphate levels to hematoporphyrin derivative-induced photosensitization in R3230 AC mammary tumors. Cancer Res 46: 211–217.
Kessel D (1986) Sites of photosensitization by derivatives of hematoporphyrin. Photochem. Photobiol. 44: 489–493.
Kosaki T, Saka T (1956) Studies on the affinities of cells and their formative elements for porphyrin bodies. Mie Med J 6: 55–64.
Moan J, Johannessen JV, Christensen T, Espevik T, McGhie JB (1982) Porphyrinsensitized photoinactivation of human cells in vitro. Am J Pathol 109: 184–192.
Moreno G, Salet C (1985) Cytotoxic effects following microirradiation of cultured cells sensitized with haematoporphyrin derivative. Int J Radiat Biol 47: 383–386.
Moreno G, Atlante A, Salet C, Santus R, Vinzens F (to be published) Photosensitivity of DNA replication and respiration to haematoporphyrin derivative (Photofrin II) in mammalian CV-1 cells. Int J Radiat Biol.
Salet C, Moreno G (1981) Photodynamic effects of haematoporphyrin on respiration and calcium uptake in isolated mitochondria. Int J Radiat Biol 39: 227–230.
Salet C, Moreno G, Vinzens F (1983) Effects of photodynamic action on energy coupling of uptake in liver mitochondria. Biochem Biophys Res Comm 115: 76–81.
Salet C, Moreno G, Vever-Bizet C, Brault D (1984) Anoxic photodamage in the presence of porphyrins: evidence for the lack of effects on mitochondrial membranes. Photochem Photobiol 40: 145–147.
Salet C, Moreno G, Bazin M, Santus R (1986) Oxic and anoxic photodamage triggered at the subcellular level by the synergistic action of hematoporphyrin derivative and nitroimidazoles. Int J Radiat Biol 50: 295–303.
Salet C, Passarella S, Quagliariello E (1987) Effects of selective irradiation on mammalian mitochondria. Photochem Photobiol 45: 433–438.
Sandberg S (1981) Protoporphyrin-induced photodamage to mitochondria and lysosomes from rat liver. Clin Chim Acta 111: 55–60.
Sandberg S, Romslo I (1980) Porphyrin-sensitized photodynamic damage of isolated rat liver mitochondria. Biochim Biophys Acta 593: 187–195.
Sandberg S, Romslo I (1981) Phototoxicity of protoporphyrin as related to its subcellular localization in mice livers after short-term feeding with griseofulvin. Biochem J 198: 67–74.
Sandberg S, Romslo I, Hovding G, Bjorndal T (1982) Porphyrin-induced photodamage as related to the subcellular localization of the porphyrins. Acta Dermatovener 100: 75–80.
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© 1988 Springer- Verlag Berlin Heidelberg
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Salet, C. (1988). Porphyrin Photosensitization of Mitochondria. In: Moreno, G., Pottier, R.H., Truscott, T.G. (eds) Photosensitisation. NATO ASI Series, vol 15. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73151-8_25
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DOI: https://doi.org/10.1007/978-3-642-73151-8_25
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