Effects of Cobalt, Indium, and Nickel on 7-Ethoxycoumarin-O-Deethylase in Mice
Cobaltous chloride is known to initiate a degradation of the hepatic microsomal cytochrome P450 content and consequently to depress the metabolic efficacy for the most xenobiotics. However, in mice after pretreatment with cobalt a paradox effect is observed with respect to metabolism of coumarin derivatives: the molecular activities towards coumarins are enhanced up to fourfold (Legrum et al. 1979; Hertle and Legrum 1980). In the living animal this increase overcompensates the loss of cytochrome P450, leading to a higher metabolism of coumarins than in controls (Legrum and Frahseck 1982). Similar inducing effects were observed after pretreatment with indium sulfate (Strack 1984; Mangoura et al. 1986).
KeywordsNickel Dioxide Cobalt Electrophoresis Warfarin
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- Hertle DR, Legrum W (1980) Metabolism of coumarins and benzo(a)pyrene in cobalt-pretreated mice. Naunyn-Schmiedeberg’s Arch Pharmacol 311[Suppl.]:R21Google Scholar
- Kling, L. Legrum W. Netter KJ (1985) Induction of liver cytochrome P-450 in mice by warfarin. Comparison of warfarin-, phenobarbitone-, and cobalt-induced hepatic microsomal protein patterns by PAGE after partial purification on octylsepharose CL-4B. Biochem Pharmacol 34:85–91PubMedCrossRefGoogle Scholar
- Mangoura SA, Legrum W, Netter KJ (1986) Indium pretreatment of mice changes the activity and the isoenzyme pattern of cytochrome P-450 sex dependently. 10th European Workshop on Drug Metabolism, GuildfordGoogle Scholar
- Netter KJ, Mangoura SA, Legrum W (1987) Cobalt-type activation of Coh locus-mediated microsomal metabolism of coumarin derivatives in mice. IUPHAR 10th International Congress of Pharmacology, SydneyGoogle Scholar
- Strack A (1984) Beeinflussung des hepatischen mischfunktionellen Monooxygenasesystems durch das Schwermetall Indium. Thesis, University of MarburgGoogle Scholar