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Detection of HLA Class II Restriction Fragment Length Polymorphisms in Multiple Sclerosis Using Pooled DNA Samples

  • R. N. S. Heard
  • I. A. Dodi
  • A. W. Downie
  • D. A. Francis
  • J. E. C. Hern
  • J. R. Batchelor
  • W. I. McDonald
  • R. L. Lechler
Conference paper

Abstract

There is by now strong evidence that susceptibility to multiple sclerosis (MS) is influenced by one or more genetic factors [1]. The familial aggregation of MS, the higher concordance rate between monozygotic than dizygotic twins and the association with serologically typed alleles encoded within the major histocompatibility complex (MHC) all provide convincing data in support of this hypothesis. HLA typing of Causasian populations of northern European ancestry with high levels of MS, in many parts of the world, has consistently shown a higher than expected prevalence of the extended haplotype HLA A3, B7, DR2, the association with DR2 being stronger than with the class I gene products. HLA DR2 can be further divided by means of cellular proliferation assays into five distinct HLA-D clusters [2] and it can be demonstrated that MS is associated particularly with the most common subtype, Dw 2 [3]. It is not yet clear whether Dw subtypes of the serologically defined DR specificities represent separate loci within the MHC or are defined by the interaction of, for instance, DR and DQ specificities [4]. In a recent study in the Grampian region of northeast Scotland, Francis et al. [5] found that DQwl was more strongly associated with MS than was DR2, although the frequency with which it was found in normal families was also increased.

Keywords

Multiple Sclerosis Major Histocompatibility Complex Restriction Fragment Length Polymorphism Multiple Sclerosis Patient Control Pool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • R. N. S. Heard
    • 1
    • 2
  • I. A. Dodi
    • 1
  • A. W. Downie
    • 3
  • D. A. Francis
    • 2
  • J. E. C. Hern
    • 3
  • J. R. Batchelor
    • 1
  • W. I. McDonald
    • 2
  • R. L. Lechler
    • 1
  1. 1.Department of ImmunologyRoyal Postgraduate Medical School, Hammersmith HospitalLondonUK
  2. 2.Institute of Neurology, Queen SquareLondonUK
  3. 3.Departments of Medicine and NeurologyAberdeen Royal InfirmaryAberdeenUK

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