Characterization of a 42 kDa Rhoptry Antigen of Toxoplasma Gondii

  • Jean François Dubremetz
  • Abderrahim Sadak
  • Zoubida Taghy
  • Bernard Fortier
Part of the NATO ASI Series book series (volume 11)

Abstract

The rhoptries of Sporozoan zoites are a set of intracellular organelles supposed to be secretory and to play a role during the invasion of the host cell by these parasites. A prerequisite to the determination of their function is the identification of the nature and properties of their contents. Little data have been obtained so far in this field except in the genus PIasmodium were a 235 kDa protein in P. yoelii (Holder & Freeman 1981) and 2 proteins of 140 and 80 kDa in P. falciparum (Holder, Freeman, Uni & Aikawa 1985; Campbell, Miller, Hudson, Franco & Andriziak 1984) have been identified in the rhoptries of erythrocytic merozoites by monoclonal antibodies (McAb). Schwartzman (1986) has obtained McAbs specific for the rhoptries of Toxoplasma gondii tachyzoites but has not identified the corresponding antigens. We report here on the McAbs characterization of a 42 kDa protein located in the rhoptries of tachyzoites and bradyzoites of T. gondii.

Keywords

Fractionation Methionine Malaria Plasmodium Dithiothreitol 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • Jean François Dubremetz
    • 1
  • Abderrahim Sadak
    • 1
  • Zoubida Taghy
    • 1
  • Bernard Fortier
    • 1
  1. 1.Unité de Biologie et Biochimie Parasitaires et FongiquesINSERM U 42Villeneuve d’AscqFrance

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