A New Intermediate in the Photocycle of Bacteriorhodopsin
The retinal-binding protein Bacteriorhodopsin in the purple membrane of Halobacteria acts as a “light-driven proton pump”. This function is controlled by a chromophoric group which contains a retinal molecule. This is bound to a lysine residue of the protein via a Schiff Base (SB) linkage. On illumination the chromophore runs through various intermediate states and under normal conditions is reconstituted within a few milliseconds. This photochemically induced and completely reversible reaction (photocycle) initiates the proton pumping mechanism /1/.
KeywordsPump Beam Cyclic Reaction Protein State Resonance Raman Purple Membrane
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