A New Intermediate in the Photocycle of Bacteriorhodopsin

  • R. Diller
  • M. Stockburger
Part of the Springer Proceedings in Physics book series (SPPHY, volume 20)


The retinal-binding protein Bacteriorhodopsin in the purple membrane of Halobacteria acts as a “light-driven proton pump”. This function is controlled by a chromophoric group which contains a retinal molecule. This is bound to a lysine residue of the protein via a Schiff Base (SB) linkage. On illumination the chromophore runs through various intermediate states and under normal conditions is reconstituted within a few milliseconds. This photochemically induced and completely reversible reaction (photocycle) initiates the proton pumping mechanism /1/.


Pump Beam Cyclic Reaction Protein State Resonance Raman Purple Membrane 
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  1. 1.
    D. Oesterhelt, W. Stoeckenius: Proc. Natl. Acad. Sci., USA 70, 2853 (1974) W. Stoeckenius, R.A. Bogomolni: Ann. Rev. Biochem. 51, 587 (1982)ADSCrossRefGoogle Scholar
  2. 2.
    R.H. Lozier, R.A. Bogomolni, W. Stoeckenius: Biophys.J. 15, 955 (1975)CrossRefGoogle Scholar
  3. 3.
    S.O. Smith, J. Lugtenburg, R.A. Mathies: J. Membrane Biol. 85, 95 (1985)CrossRefGoogle Scholar
  4. 4.
    M. Stockburger, T. Alshuth, D. Oesterhelt, W. Gärtner: In Advances of Spectroscopy, ed. by R.J.H. Clark and R.E. Hester, Vol. 13, Spectroscopy of Biological Systems, J. Wiley a. Sons, (1986) p. 483Google Scholar
  5. 5.
    T. Alshuth, M. Stockburger: Photochem. Photobiol. 43, 55 (1986)CrossRefGoogle Scholar
  6. 6.
    U. Fischer, D. Oesterhelt: Biophys. J. 28, 211 (1979) M. Engelhard, K. Gerwert, B. Hess, W. Kreutz, F. Siebert: Biochemistry 24, 400 (1985)CrossRefGoogle Scholar
  7. 7.
    P. Hildebrandt, M. Stockburger: Biochemistry 6, 5539 (1984)CrossRefGoogle Scholar
  8. 8.
    P. Tavan, K. Schulten: Biophys. J. 50, 81 (1986)CrossRefGoogle Scholar
  9. 9.
    W.V. Sherman, R.R. Eicke, S.R. Stafford, F.M. Wasacz: Photochem. Photobiol. 30, 727Google Scholar
  10. 10.
    W. Mäntele: Ph.D. Thesis, University of Freiburg, Freiburg, F.R.G. (1982)Google Scholar
  11. 11.
    T. Alshuth: Ph.D. Thesis, University of Göttingen, Göttingen, F.R.G. (1985)Google Scholar
  12. 12.
    Q. Li, R. Govindjee, T.G. Ebrey: Proc. Natl. Acad. Sci. USA, 81, 7079 (1984)ADSCrossRefGoogle Scholar
  13. 13.
    S.O. Smith, J.A. Pardoen, P.P.J. Mulder, B. Curry, J. Lugtenburg, R. Mathies: Biochemistry 22, 6141 (1983)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • R. Diller
    • 1
  • M. Stockburger
    • 1
  1. 1.Max-Planck-Institut für Biophysikalische ChemieGöttingenFed. Rep. of Germany

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