Two Kinds of Bacteriorhodopsin Analogues Synthesized from Naphthylretinal

  • F. Tokunaga
  • M. Takao
  • T. Iwasa
  • K. Tsujimoto
Part of the Springer Proceedings in Physics book series (SPPHY, volume 20)


Two kinds of bacteriorhodopsin analogues were formed with all-trans-naphthylretinaland bacterioopsin. One has an absorption maximum at 503 nm (Np-bR503) and the other, at 442 nm (Np-bR442). An equimolar mixture of naphthylretinal and bacterioopsin produced Np-bR442, while successive addition of naphthylretinal gave Np-bR503. The binding sites of naphthylretinal in Np-bR442 and in Np-bR503 were the same as retinal in the native bacteriorhodopsin. The CD spectrum of Np-bR503 shows characteristic exciton CD bands, but that of Np-bR442, only a positive band. Np-bRs produced own batho- and meta-intermediates different from each other. The pH increase and temperature elevation change Np-bR503 to Np-bR442 reversibly. Solubilization of Np-bR503 in Triton X-100 also changes Np-bR503 to Np-bR442. These observations indicate that Np-bR503 and Np-bR442 are in equilibrium.


Isosbestic Point Absorbance Increase Purple Membrane Original Pigment Dark Reversion 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • F. Tokunaga
    • 1
  • M. Takao
    • 1
  • T. Iwasa
    • 1
  • K. Tsujimoto
    • 2
  1. 1.Department of Physics, Faculty of ScienceTohoku UniversityAobayama, Sendai 980Japan
  2. 2.Department of Material ScienceUniversity of ElectrocommunicationsChofu 182Japan

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