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Two Kinds of Bacteriorhodopsin Analogues Synthesized from Naphthylretinal

  • F. Tokunaga
  • M. Takao
  • T. Iwasa
  • K. Tsujimoto
Part of the Springer Proceedings in Physics book series (SPPHY, volume 20)

Abstract

Two kinds of bacteriorhodopsin analogues were formed with all-trans-naphthylretinaland bacterioopsin. One has an absorption maximum at 503 nm (Np-bR503) and the other, at 442 nm (Np-bR442). An equimolar mixture of naphthylretinal and bacterioopsin produced Np-bR442, while successive addition of naphthylretinal gave Np-bR503. The binding sites of naphthylretinal in Np-bR442 and in Np-bR503 were the same as retinal in the native bacteriorhodopsin. The CD spectrum of Np-bR503 shows characteristic exciton CD bands, but that of Np-bR442, only a positive band. Np-bRs produced own batho- and meta-intermediates different from each other. The pH increase and temperature elevation change Np-bR503 to Np-bR442 reversibly. Solubilization of Np-bR503 in Triton X-100 also changes Np-bR503 to Np-bR442. These observations indicate that Np-bR503 and Np-bR442 are in equilibrium.

Keywords

Isosbestic Point Absorbance Increase Purple Membrane Original Pigment Dark Reversion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • F. Tokunaga
    • 1
  • M. Takao
    • 1
  • T. Iwasa
    • 1
  • K. Tsujimoto
    • 2
  1. 1.Department of Physics, Faculty of ScienceTohoku UniversityAobayama, Sendai 980Japan
  2. 2.Department of Material ScienceUniversity of ElectrocommunicationsChofu 182Japan

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