Advertisement

Stimulation of In Vitro H+ Transport in Zucchini Microsomes by the Ether Lipid Platelet Activating Factor and a Soluble Protein

  • Günther F. E. Scherer
  • Georg Martiny-Baron
Conference paper
Part of the NATO ASI Series book series (volume 10)

Summary

Platelet activating factor (=PAF) stimulates H+ transport in microsomes from zucchini hypocotyl (1) and ATP hydrolysis. The apparent membrane permeability for protons in membrane vesicles is not changed by PAF and in ATP hydrolysis rather vmax than the Km is changed by PAF. PAF stimulation of H+ transport is dependent on the presence of a protein factor which can be removed from the membranes. The properties of PAF-dependent protein factor in DEAE-Sephacel chromatography are very similar to a protein kinase. PAF stimulation of ATP hydrolysis and H+ transport is found in tonoplast and plasma membranes. A possible role for the PAF-dependent factor analogous to animal protein kinase C in plant hormone action is postulated.

Keywords

ATPase Activity Platelet Activate Factor Transport Assay Sucrose Step Gradient Transport Stimulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature

  1. (1).
    Scherer, G.F.E. (1985) Biochem. Biophys. Res. Comm. 133, 1160–1167PubMedCrossRefGoogle Scholar
  2. (2).
    Demopoulos, C.A., Pinckard, R.N., Hanahan, D.J. (1979) J. Biol. Chem. 254, 9355–9358PubMedGoogle Scholar
  3. (3).
    Vargaftig, B.B., Benveniste, J. (1983) Trends in Pharm. Sci. 4, 341–343CrossRefGoogle Scholar
  4. (4).
    Conrad, G.W., Rink, T.J. (1986) J. Cell Biol. 103, 439–450PubMedCrossRefGoogle Scholar
  5. (5).
    Fisher, R.A., Shukla, S.D., Debuysere, M.S., Hanahan, D.J., Olson, M.S. (1984) J. Biol. Chem. 259, 8685–8688PubMedGoogle Scholar
  6. (6).
    Sugatani, J., Hanahan, D.J. (1986) Arch. Biochem. Biophys. 246, 855–864PubMedCrossRefGoogle Scholar
  7. (7).
    Scherer, G.F.E., Fischer, G. (1985) Protoplasma 129, 109–119CrossRefGoogle Scholar
  8. (8).
    Scherer, G.F.E. (1981) Planta, 151, 434–438CrossRefGoogle Scholar
  9. (9).
    Scherer, G.F.E., Martiny-Baron, G. (1985) Plant Science 41, 161–168CrossRefGoogle Scholar
  10. 10).
    Buckhout, T.J. (1982) Planta 159, 84–90CrossRefGoogle Scholar
  11. 11).
    Takai et al. (1979) J. Biol. Chem. 254, 3692–3695PubMedGoogle Scholar
  12. 12).
    Schäfer, B., et al. (1985) FEBS Lett. 187, 25–28CrossRefGoogle Scholar
  13. 13).
    Scherer, G.F.E. (1984) Planta 160, 348–356CrossRefGoogle Scholar
  14. 14).
    Nishizuka, Y. (1984) Trends in Biochem. Sci. 9, 163–166CrossRefGoogle Scholar
  15. 15).
    Hertel, R. (1979) Plant Organelles. Methodological Surveys (B) Biochemistry vol. 9 pp. 173–183. E. Reid, ed. Halsted Press, New York Chichester TorontoGoogle Scholar
  16. 16).
    Kutschera, U., Schopfer, P. (1985) Planta 163, 483–493CrossRefGoogle Scholar
  17. 17).
    Kutschera, U., Schopfer, P. (1985) Planta 163, 494–499CrossRefGoogle Scholar
  18. 18).
    Buckhout, T.J. (1984) Plant Physiol. 76, 962–967PubMedCrossRefGoogle Scholar
  19. 19).
    Buckhout, T.J., Young, K.A., Low, P.S., Morre, D.J. (1981) Plant Physiol. 68, 512–515PubMedCrossRefGoogle Scholar
  20. 20).
    Ray, P.M., Dohrmann, U., Hertel, R. (1977) Plant Physiol. 59, 537–546Google Scholar
  21. 21).
    Muallem, S., Schoeffield, M., Pandol, S., Sachs, G. (1985) Proc. Natl. Acad. Sci. USA 82, 4433–4437PubMedCrossRefGoogle Scholar
  22. 22).
    Boss, W.F., Massel, M.O. (1985) Biochem. Biophys. Res. Comm. 132, 1018–1023PubMedCrossRefGoogle Scholar
  23. 23).
    Strasser, H., Hoffmann, C., Grisebach, H., Matern, U., (1986) Z. Naturforsch. 41 c, 717–724Google Scholar
  24. 24).
    Yoshida, S. (1979) Plant Physiol, 64, 241–246PubMedCrossRefGoogle Scholar
  25. 25).
    Scherer, G.F.E., Morre, D.J. (1978) Plant Physiol. 62, 933–937PubMedCrossRefGoogle Scholar
  26. 26).
    Sze, H. (1985) Annu. Rev. Plant Physiol. 36, 175–208CrossRefGoogle Scholar
  27. 27).
    Zbell, B. (1983) Ph. D. thesis, Berlin, Free UniversityGoogle Scholar
  28. 28).
    Hertel, R. (1983) Z. Pflanzenphysiol. 112, 53–67Google Scholar
  29. 29).
    De la Fuente, R.K. (1984) Plant Physiol. 76, 342–346CrossRefGoogle Scholar
  30. 30).
    Gabathuler, R., Cleland, R.E. (1985) Plant Physiol. 79, 1080–1085PubMedCrossRefGoogle Scholar
  31. 31).
    Scherer, G.F.E. (1984) Planta 161, 394–397CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • Günther F. E. Scherer
    • 1
  • Georg Martiny-Baron
    • 1
  1. 1.Botanisches Institut der Universität BonnBonn 1Germany

Personalised recommendations