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Receptor Analysis: Data Processing in the Presence of Nonspecific Binding Sites

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Investigative Urology 2

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Abstract

In biochemical receptor analysis 2 parameters are of particular interest: the concentration of receptor binding sites and the dissociation constant of the receptor-ligand complex. For this purpose cell fractions or intact cells containing the receptor are incubated in vitro with radioactive ligand until equilibrium is achieved:

$${\text{L}} + {\text{R}} + \rightleftarrows {\text{RL}} + {\text{BL}}$$
((1))

L = free radioactive ligand, R = free receptor, B = free nonspecific binder, RL = radioactive receptor-ligand complex, BL = radioactive complex of ligand and non-specific binder.

Supported by the Deutsche Forschungsgemeinschaft (Mo 315/3-2)

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References

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Author information

Authors and Affiliations

  1. Children’s Hospital, University of Tübingen, D-7400, Tübingen, Germany

    H. Moeller, Ch. Fusch, A. Kuch & N. Manolopoulos

  2. Department of Anatomy, University of Tübingen, D-7400, Tübingen, Germany

    G. Oettling

Authors
  1. H. Moeller
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  2. Ch. Fusch
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  3. A. Kuch
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  4. N. Manolopoulos
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  5. G. Oettling
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Editor information

Editors and Affiliations

  1. Urologische Klinik und Poliklinik, im Klinikum der Johannes-Gutenberg-Universität, Langenbeckstraße 1, D-6500, Mainz, Germany

    Günther H. Jacobi

  2. Abteilung Urologie, Knappschaftskrankenhaus Bardenberg, Dr.-Hans-Böckler-Platz 1, D-5102, Würselen 1, Germany

    Herbert Rübben

  3. Urologische Klinik, Zentralklinikum, Stenglinstraße, D-8900, Augsburg, Germany

    Rolf Harzmann

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© 1987 Springer-Verlag Berlin Heidelberg

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Moeller, H., Fusch, C., Kuch, A., Manolopoulos, N., Oettling, G. (1987). Receptor Analysis: Data Processing in the Presence of Nonspecific Binding Sites. In: Jacobi, G.H., Rübben, H., Harzmann, R. (eds) Investigative Urology 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72735-1_17

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  • DOI: https://doi.org/10.1007/978-3-642-72735-1_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72737-5

  • Online ISBN: 978-3-642-72735-1

  • eBook Packages: Springer Book Archive

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