Summary
We describe the development of a sensitive radioimmunoassay (RIA) for erythropoietin (EPO) which uses recombinant human EPO-derived reagents. Evidence of assay validity includes parallelism of standard and test samples (human sera and impure preparations of urinary and recombinant EPO), correlation of serum EPO levels with clinical condition and agreement of RIA and in vivo bioassay estimates of EPO. In addition,EPO levels determined using the present assay agree well with estimates obtained when the same test samples are assayed in a RIA using human urinary EPO-derived reagents. These results indicate that the RIA employing recombinant EPO reagents has the necessary sensitivity and specificity to assay the natural hormone.
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© 1987 Springer-Verlag Berlin Heidelberg
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Egrie, J.C., Lane, J. (1987). Development of a Radioimmunoassay for Erythropoietin Using Recombinant Erythropoietin-Derived Reagents. In: Rich, I.N. (eds) Molecular and Cellular Aspects of Erythropoietin and Erythropoiesis. NATO ASI Series, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72652-1_29
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DOI: https://doi.org/10.1007/978-3-642-72652-1_29
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