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Erythropoiesis and Macrophage Subsets in Medullary and Extramedullary Sites

  • J. P. de Jong
  • P. G. J. Nikkels
  • A. H. Piersma
  • R. E. Ploemacher
Conference paper
Part of the NATO ASI Series book series (volume 8)

Summary

Morphological and functional evidence exists that macrophages support in vivo erythropoiesis. Using in vitro cultured primary adherent cells from murine bone marrow as antigenic source, we have prepared a hybridoma cell line secreting a monoclonal antibody (mAb) to reticular cells of bone marrow and non-lymphoid domains in the spleen. The mAb (α-ER-HR3) also binds to some elongated cells in the subcutaneous tissue and to intertubular areas of the renal medulla. In addition, the ER-HR3 antigen is expressed on some reticular cells in the capsular sinuses and paracortex of lymph nodes and in the lamina propria of the ilium and colon, with increasing expression towards the distal ileum. The expression of the ER-HR3 antigen in non-lymphoid domains of hemopoietic organs is proposed to be associated with adult type hemoglobin (Hb) erythropoiesis as evidenced by (1) the absence in the yolk sac, (2) the exact correlation with the presence of adult type Hb erythropoiesis, but not granulopoiesis, in fetal and neonatal liver and spleen and postnatal bone marrow and spleen, and (3) the correlation with phenylhydrazine-induced hepatic erythropoiesis in the adult liver.

Following injection of highly purified -ER-HR3 into neonatal mice, a transient decrease of hemopoietic progenitor cells, but not of day-7 and day-12 CFU-S, was observed in the bone marrow,resulting in a moderate macrocytic anemia. Injection of -ER-HR3 also limited the erythropoietin-induced 59Fe incorporation in the bone marrow of adult hypertransfused mice. The data obtained so far strongly suggest that the ER-HR3 antigen is exclusively expressed by a macrophage subset.

Keywords

Bone Marrow Murine Bone Marrow Hemopoietic Stem Cell Macrophage Subset Fibroblast Coloni 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • J. P. de Jong
    • 1
  • P. G. J. Nikkels
    • 1
  • A. H. Piersma
    • 1
  • R. E. Ploemacher
    • 1
  1. 1.Department of Cell Biology and GeneticsErasmus UniversityRotterdamThe Netherlands

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