The Nucleoskeleton: Active Site of Transcription and Replication

  • D. A. Jackson
  • P. R. Cook
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 31)

Abstract

Nuclei and chromatin are rarely studied at a physiological salt concentration since they aggregate so readily [16]. As a result, they are generally studied in the presence of “stabilizing” divalent cations under hyper- or hypotonic conditions. Such conditions are unsatisfactory for several reasons. The “stabilizing” cations activate nucleases, destroying template integrity and supercoiling, and unphysiological salt concentrations may introduce artefacts. It has been suggested that structures called variously the nuclear matrix, cage or scaffold, are the site of replication and transcription [8], but they are not seen in the micrographs of “genes in action” obtained by Miller and colleagues using hypotonic conditions [15, 14].

Keywords

EDTA Cage Leukemia Agarose Electrophoresis 

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References

  1. 1.
    Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1983) Molecular biology of the cell. Garland, New YorkGoogle Scholar
  2. 2.
    Ariga H, Sugano S (1983) J Virol 48:481–491PubMedGoogle Scholar
  3. 3.
    Cook PR (1984) EMBO J 3:1837–1842PubMedGoogle Scholar
  4. 4.
    Cook PR, Brazell IA (1976) J Cell Sci 22:287–302PubMedGoogle Scholar
  5. 5.
    Cook PR, Lang J, Hayday A, Lania L, Fried M, Chiswell DJ, Wyke JA (1982) EMBO J 1:447–452PubMedGoogle Scholar
  6. 6.
    Jackson DA, McCready SJ, Cook PR (1981) Nature 292:552–555PubMedCrossRefGoogle Scholar
  7. 7.
    Jackson DA, Caton AJ, McCready SJ, Cook PR (1982) Nature 296:366–368PubMedCrossRefGoogle Scholar
  8. 8.
    Jackson DA, McCready SJ, Cook PR (1984) J Cell Sci Suppl 1:59–79PubMedGoogle Scholar
  9. 9.
    Jackson DA, Cook PR (1985) EMBO J 4:913–918PubMedGoogle Scholar
  10. 10.
    Jackson DA, Cook PR (1985) EMBO J 4:919–925PubMedGoogle Scholar
  11. 11.
    Jackson DA, Cook PR (1986) EMBO J 5:1403–1410PubMedGoogle Scholar
  12. 12.
    Li JJ, Kelly TJ (1984) Proc Natl Acad Sci USA 81:6973–6977PubMedCrossRefGoogle Scholar
  13. 13.
    Manley JL, Fire A, Cano A, Sharp PA, Gefter ML (1980) Proc Natl Acad Sci USA 77:3855–3859PubMedCrossRefGoogle Scholar
  14. 14.
    McKnight SL, Miller OL (1979) Cell 17:551–563PubMedCrossRefGoogle Scholar
  15. 15.
    Miller OL, Beattie BR (1969) Science 164:955–957PubMedCrossRefGoogle Scholar
  16. 16.
    Ohlenbusch HH, Olivera BM, Tuan D, Davidson N (1967) J Mol Biol 25:299–315PubMedCrossRefGoogle Scholar
  17. 17.
    Robinson SI, Nelkin BD, Vogelstein B (1982) Cell 28:99–106PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • D. A. Jackson
    • 1
  • P. R. Cook
    • 1
  1. 1.Sir William Dunn School of PathologyUniversity of OxfordOxfordEngland

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