The Nucleoskeleton: Active Site of Transcription and Replication

  • D. A. Jackson
  • P. R. Cook
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 31)


Nuclei and chromatin are rarely studied at a physiological salt concentration since they aggregate so readily [16]. As a result, they are generally studied in the presence of “stabilizing” divalent cations under hyper- or hypotonic conditions. Such conditions are unsatisfactory for several reasons. The “stabilizing” cations activate nucleases, destroying template integrity and supercoiling, and unphysiological salt concentrations may introduce artefacts. It has been suggested that structures called variously the nuclear matrix, cage or scaffold, are the site of replication and transcription [8], but they are not seen in the micrographs of “genes in action” obtained by Miller and colleagues using hypotonic conditions [15, 14].


Transcription Complex Replication Complex Nascent Transcript EcoRI Digestion Isotonic Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • D. A. Jackson
    • 1
  • P. R. Cook
    • 1
  1. 1.Sir William Dunn School of PathologyUniversity of OxfordOxfordEngland

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