Abstract
The reciprocal translocation between human chromosomes 9 and 22, termed the Philadelphia chromosome (Ph 1), is observed in more than 90% of patients with chronic myelogenous leukemia. This translocation fuses sequences from a variable distance 5′ to the c-abl locus on chromosome 9 to sequences in a breakpoint cluster region (bcr) on chromosome 22. The appearance of the Ph 1 chromosome is correlated with the production of a novel 8.7-kb RNA transcript containing both bcr and c-abl sequences as well as with a 210-kd phosphoprotein (p210c-abl) representing non-abl polypeptide sequences fused to c-abl-derived sequences. Antibodies prepared to a number of different c-abl domains and to bcr determinants were employed to characterize the normal and altered c-abl gene products. By combining a variety of cDNA cloning techniques, we have isolated bcr/abl clones representing 8.7 kb of contiguous mRNA sequence.
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© 1987 Springer-Verlag Berlin Heidelberg
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Mes-Masson, AM., McLaughlin, J., Witte, O. (1987). Molecular Cloning and Serological Characterization of an Altered c-abl Gene Product Produced in Ph 1 CML Patients. In: Neth, R., Gallo, R.C., Greaves, M.F., Kabisch, H. (eds) Modern Trends in Human Leukemia VII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72624-8_35
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DOI: https://doi.org/10.1007/978-3-642-72624-8_35
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