Abstract
The reciprocal translocation between human chromosomes 9 and 22, termed the Philadelphia chromosome (Ph 1), is observed in more than 90% of patients with chronic myelogenous leukemia. This translocation fuses sequences from a variable distance 5′ to the c-abl locus on chromosome 9 to sequences in a breakpoint cluster region (bcr) on chromosome 22. The appearance of the Ph 1 chromosome is correlated with the production of a novel 8.7-kb RNA transcript containing both bcr and c-abl sequences as well as with a 210-kd phosphoprotein (p210c-abl) representing non-abl polypeptide sequences fused to c-abl-derived sequences. Antibodies prepared to a number of different c-abl domains and to bcr determinants were employed to characterize the normal and altered c-abl gene products. By combining a variety of cDNA cloning techniques, we have isolated bcr/abl clones representing 8.7 kb of contiguous mRNA sequence.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ben-Neriah Y, Bernards A, Paskind M, Daley GQ, Baltimore D (1986a) Cell 44:577–586
Ben-Neriah Y, Daley GQ, Mes-Masson A-M, Witte ON, Baltimore D (1986b) Science (to be published)
Cananni E, Gale RP, Steinder-Saltz D, Berrebi A, Aghai E, Januszewicz E (1984) Lancet 1:593–595
Collins S, Kubonishi I, Myoshi I, Groudine MT (1984) Science 225:72–74
Goff SP, Gilboa E, Witte ON, Baltimore D (1980) Cell 22:777–785
Groffen J, Stephenson JR, Heisterkamp N, Klein A de, Bartram A, Bartram CR, Grosveld G (1984) Cell 36:93–99
Grosveld G, Verwoerd T, Agthoven T van, Klein A de, Ramachandran KL, Heisterkamp N, Stam K, Groffen J (1986) Mol Cell Biol 6:607–616
Heisterkamp N, Stephenson JR, Groffen J, Hansen PF, Klein A de, Bartram CR, Grosveld G (1983) Nature 306:239–242
Heisterkamp N, Stam K, Groffen J, Klein A de, Grosveld G (1985) Nature 315:758–761
Koeffler MD, Golde DW (1981) N Engl J Med 304:1201–1209, 1269–1274
Konopka JB, Witte ON (1985) Mol Cell Biol 5:3116–3123
Konopka JB, Watanabe SM, Witte ON (1984a) Cell 37:1035–1042
Konopka JB, Davis RL, Watanabe SM, Ponticelli AS, Schiff-Maker L, Rosenberg N, Witte ON (1984b) J Virol 51:223–232
Prywes R, Foulkes JG, Rosenberg N, Baltimore D (1983) Cell 34:569–579
Reddy EP, Smith MJ, Srinivasan A (1983) Proc Natl Acad Sci USA 80:3623–3627
Rosenberg N, Baltimore D (1976) J Exp Med 147:1126–1141
Rosenberg N, Baltimore D, Scher CD (1975) Proc Natl Acad Sci USA 75:3974–3978
Rosenberg N, Clark DR, Witte ON (1980) J Virol 36:766–774
Rowley JD (1973) Nature 243:290–291
Shtivelman E, Lifshitz B, Gale RP, Canaani E (1985) Nature 315:550–554
Wang JYJ, Ledley F, Goff S, Lee R, Groner Y, Baltimore D (1984) Cell 36:349–356
Whitlock CA, Witte ON (1986) Adv Immunol (to be published)
Witte ON, Rosenberg N, Baltimore D (1979) Nature 281:396–398
Witte ON, Dasgupta A, Baltimore D (1980) Nature 283:826–831
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Mes-Masson, AM., McLaughlin, J., Witte, O. (1987). Molecular Cloning and Serological Characterization of an Altered c-abl Gene Product Produced in Ph 1 CML Patients. In: Neth, R., Gallo, R.C., Greaves, M.F., Kabisch, H. (eds) Modern Trends in Human Leukemia VII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72624-8_35
Download citation
DOI: https://doi.org/10.1007/978-3-642-72624-8_35
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-17754-8
Online ISBN: 978-3-642-72624-8
eBook Packages: Springer Book Archive