Abstract
In situ hybridization techniques are currently the most direct way of determining the localization and quantification of repetitive sequences or unique genes, e.g. oncogenes, in tissues or on chromosomes. This method involves the annealing of labeled polynu-cleotide sequences to chromosomal or cellular preparations whose DNA or RNA has been denatured (or otherwise exposed) to enable hybridization with the labeled probe. Along with autoradiographic techniques [5, 6], the use of nonisotopic in situ hybridization methods has brought a better resolution of the signal and a considerable shortening of the procedure.
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Ambros, P.F., Bartram, C.R., Haas, O.A., Karlic, H.I., Gadner, H. (1987). Nonisotopic In Situ Hybridization for Mapping Oncogenic Sequences. In: Neth, R., Gallo, R.C., Greaves, M.F., Kabisch, H. (eds) Modern Trends in Human Leukemia VII. Haematology and Blood Transfusion / Hämatologie und Bluttransfusion, vol 31. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72624-8_30
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DOI: https://doi.org/10.1007/978-3-642-72624-8_30
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