An Inert Mixture for Solubilizing Lipophilic Drugs in Cell Culture Assays
The search for inert solvent mixtures to assay lipophilic molecules in cell cultures has been carried out in order to test aquamimetic compounds such as dimethyl sulfoxide (DMSO) or nonionic surfactants such as sorbitan derivatives and macrogol esters in the absence or in the presence of ethanol or glycols. However, some cytotoxic effects have been reported for these solvent mixtures which are largely employed for pharmaceutical purposes. Polyoxyethyleneglycerol triricinoleate (Cremophor EL) holds an important role among the solubilizers best tolerated in vivo. For instance, it emulsifies fat soluble vitamins and cyclosporin A in aqueous-alcoholic solution, without producing any apparent cell injury. On the other hand, anaphylactoid reactions (Dye and Watkins 1980), transient hematological and biochemical disturbances (Padfield and Watkins 1977), and unusual hyper-lipidemias (Forrest et al. 1977) have been reported when Cremophor EL was used as aqueous solution for intravenous injection of anesthetic drugs, e.g., Althesin (alphaxalone and alphadolone; Forrest et al. 1977) and propanidid (Watkins 1979).
KeywordsNonionic Surfactant Lipophilic Drug Anaphylactoid Reaction Ethanol Mixture Lipophilic Molecule
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