Abstract
The atomic force microscope, AFM (3), images hard surfaces at atomic resolution but its application to soft biological samples is still a great challenge (4, 5, 6, 7, 8). An important characteristic of the AFM is that it allows imaging of biological material not only in air or vacuum but also in aqueous solutions without any preceeding fixation or staining. We have applied the AFM to the analysis of the two-dimensional crystals of Na+/K+-ATPase, which we have previously characterized by electron microscopy following negative staining (11) or cryo-electron microscopy (10). The present observations demonstrate that the lipid regions of Na+/K+-ATPase membranes can be imaged unstained in aqueous solutions at molecular resolution.
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© 1994 Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt
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Maunsbach, A.B., Thomsen, K. (1994). Atomic Force Microscopy of Crystallized Na+/K+-ATPase. In: Bamberg, E., Schoner, W. (eds) The Sodium Pump. Steinkopff. https://doi.org/10.1007/978-3-642-72511-1_54
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DOI: https://doi.org/10.1007/978-3-642-72511-1_54
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