Abstract
Digitalis substances (cardenolides and bufodienolides) are potent and specific inhibitors of the cell membrane Na,K-ATPase. Cardenolides are synthetized in a number of plants and bufodienolides are endogenous substances in certain species of amphibians (1,2). The demonstration of cardiotonic steroids in lower vertebrates and of specific receptors for digitalis on the surface of most mammalian cells have suggested the existence of endogenous digitalis-like factors (EDLF) also in mammalians (2,3). Several substances with endogenous digitalis-like activity have been purified from mammalian tissues and body fluids (2,3). Recently, Hamlyn et al. have isolated from human plasma and identified by mass spectroscopy a substance that is indistinguishable from ouabain, proposing that ouabain is an endogenous digitalis-like factor (4). However, this conclusion is still object of controversies (5).
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References
Heftmann E (1970) Steroid Biochemistry. New York: Academic Press.
Lichtstein D, Samuelov S, Gati I, Wechter WJ (1992) Digitalis-like compounds in animal tissue. J Basic Clinical Physiol & Pharmac; 3: 269–370.
Goto A, Yamada K, Yagi N, Yoshioka M, Sugimoto T (1992) Physiology and pharmacology of endogenous digitalis-like factors. Pharmacol Rev; 44: 377–399.
Mathews WR, DuCharme DW, Hamlyn JM, Harris DW, Mandel F, Clark MA, Ludens JH (1991) Mass spectral characterization of an endogenous digitalis-like factor from human plasma. Hypertension; 17: 930–935.
Kelly RA, Smith TW (1992) Is ouabain the endogenous digitalis? Circulation; 86: 694–697.
Balzan S, Ghione S, Biver P, Gazzetti P, Montali U (1991) Partial purification of endogenous digitalis-like compound(s) in cord blood. Clin Chem; 37: 277–281.
Montali U, Balzan S, Ghione S (1991) Purification of endogenous digitalis-like factor(s) from cord blood of neonate by immunoaffinity chromatography. Biochem Int; 25: 853–859.
Balzan S, Montali U, Genovesi-Ebert A, Biver P, Fantoni M, Ghione S (1989) Comparison between endogenous digoxin-like immunoreactivity and 86Rb uptake by erythrocytes in extracts of human plasma. Clin Sci; 77: 375–381.
Harris DW, Clark MA, Fisher JF, Hamlyn JM, Kolbasa KP, Ludens JH, DuCharme DW (1991) Development of an immunoassay for endogenous digitalis-like factor. Hypertension; 17: 936–943.
Tamura M, Lam TT, Inagami T (1988) Isolation and characterization of a specific endogenous NaK ATPase inhibitor from bovine adrenal. Biochemistry; 27: 4244–4253.
Ferrandi M, Minotti E, Salardi S, Florio M, Bianchi G, Ferrari P (1992) Ouabainlike factor in Milan hypertensive rats. Am J Physiol; 263: F739–F748.
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© 1994 Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt
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Balzan, S., Ghione, S., Pieraccini, L., Biver, P., Di Bartolo, V., Montali, U. (1994). Endogenous Digitalis-Like Factor from Umbilical Cord and Ouabain: Comparison of Biochemical Properties. In: Bamberg, E., Schoner, W. (eds) The Sodium Pump. Steinkopff. https://doi.org/10.1007/978-3-642-72511-1_135
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DOI: https://doi.org/10.1007/978-3-642-72511-1_135
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