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Comparison of E1 and E2 Conformers of Membrane Bound Na+/K+-ATPase Using Time-Resolved Phosphorescence Anisotropy Measurements

  • A. Boldyrev
  • O. Lopina
  • A. Rubtsov
  • D. McStay
  • L. Yang
  • P. Quinn

Abstract

Na+/K+-ATPase is known to form oligomeric complexes in the membrane bilayer. On the other hand, there are strong evidence that monomelic form possesses all of the main hydrolytic and transport properties of the Na+-pump. This raises the question as to whether formation of oligomeric complexes is a the necessary step in the biochemical processes associated with the translocation of cations across the membrane or simply an architectural feature of the intercalation of the proteins into the phospholipid bilayer. We have addressed this question by measuring the rotational relaxation of the ATPase in different conformational states of the pump cycle using flash-photolysis methods.

Keywords

Oligomeric Form Salt Gland Rotational Correlation Time Rotational Relaxation Oligomeric Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Boldyrev A, Fedosova N, Lopina O (1991) The mechanism of the modifying effect of ATP on Na+/K+-ATPase. Biomed Sci 2: 450–454PubMedGoogle Scholar
  2. 2.
    Mimura K, Matsui H, Takagi T, Hayashi Y (1993) Change in oligomeric structure of solubilized Na+/K+-ATPase induced by octaethylene glycol dodecyl ether, phosphatidylserine and ATP. Biochim Biophys Acta 1145: 63–74PubMedCrossRefGoogle Scholar
  3. 3.
    Yang L, Lopina O, Rubtsov A, McStay D, Boldyrev A, Quinn P (1993) Rotational dynamics of membrane-bound Na+/K+-ATPase revealed by the time-resolved phosphorescence anisotropy. Biochem J (in press)Google Scholar

Copyright information

© Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1994

Authors and Affiliations

  • A. Boldyrev
    • 2
    • 1
  • O. Lopina
    • 2
    • 1
  • A. Rubtsov
    • 2
    • 1
  • D. McStay
    • 2
    • 1
  • L. Yang
    • 2
    • 1
  • P. Quinn
    • 2
    • 1
  1. 1.King’s College LondonLondonUK
  2. 2.Moscow State UniversityMoscowRussia

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