Immunohistochemical evidence for a neurosecretory and neurotransmitter function of CDD/ANP-immunoreactive neurons in molluscs

  • M. Reinecke
  • H. Back
  • P. Jacobs
  • R. Schipp
Conference paper

Abstract

Recently, our group showed (5,6,7) that cardiac hormones of the atrial natriuretic polypeptide (ANP)/cardiodilatin (CDD)-family (for review see 2) occur in myoendocrine cells of the hearts from representatives of all vertebrate classes. In the snail Helix pomatia (Gastropoda, Pulmonata), however, CDD/ANP-immunoreactivity was found exclusivly in neuroendocrine endings of the cardiac atrium while no CDD/ANP- immunoreactivity was present in cardiac myocytes (4,5). In correlation, no “specific” granules which characterize the cardiac myoendocrine system of all vertebrates (1,5,6,7) could be observed in heart muscle cells of the snail. Since furthermore, CDD/ANP-immunoreactivity was present in perikarya of the subesophageal ganglion and in nerve fibers of the intestinal nerve which supplies the heart, the cardiac hormone-like substances seem to constitute a neuroendocrine-cardiac axis in the snail Helix pomatia (4,5). In order to investigate this peculiar phylogenetic pattern in more detail we expanded our studies 1) by analyzing the exact distribution patterns of CDD/ANP-immunoreactive (-IR) neurons in the central ganglia of Helix pomatia and 2) by investigating the hearts and the central ganglia of other molluscan species, i.e., Aplysia depilans (Gastropoda, Opisthobranchia) and Sepia officinalis (Cephalopoda, Decabrachia) for the occurance of cardiac hormones. The study was carried out with Bouin-fixed paraffin-embedded tissue by means of immunohistochemistry applying several region-specific antisera against alpha-ANP (=CDD-28) and the peroxidase-antiperoxidase (PAP) technique.

Keywords

Polypeptide Meso Gastropoda FMRFamide Myxine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Chapeau, C., Gutkowska, J., Schiller, P.W., Milne, R.W., Thibault, G., Garcia, R., Genest, J., Cantin, M. (1985) Localization of immunoreactive synthetic atrial natriuretic factor (ANF) in the heart of various animal species. J Histochem Cyto-chem 33: 541–550CrossRefGoogle Scholar
  2. 2.
    Forssmann, W.G. (1986) Cardiac hormones. I. Review on the morphology, biochemistry and molecular biology of the endocrine heart. Eur J Clin Invest 16: 439–451PubMedCrossRefGoogle Scholar
  3. 3.
    Martin, R., Voigt, K.H. (1987) The neurosecretory system of the octopus vena cava: A neurohaemal organ. Experientia 43: 537–543CrossRefGoogle Scholar
  4. 4.
    Nehls, M., Reinecke, M., Lang, R.E., Forssmann, W.G. (1985) Biochemical and immunological evidence for a cardiodilatin-like substance in the snail neurocardiac axis. Proc Natl Acad Sci USA 82: 7762–7766PubMedCrossRefGoogle Scholar
  5. 5.
    Reinecke, M., Nehls, M., Forssmann, W.G. (1985) Phylogenetic aspects of cardiac hormones as revealed by immunocytochemistry, electronmicroscopy and bioassay. Peptides 6 (suppl 3): 321–331PubMedCrossRefGoogle Scholar
  6. 6.
    Reinecke, M., Betzier, D., Forssmann, W.G. (1987a) Immunocytochemistry of cardiac polypepide hormones (Cardiodilatin/atrial natriuretic polypeptide) in brain and hearts of Myxine glutinosa (Cyclostomata). Histochemistry 86: 233–239PubMedCrossRefGoogle Scholar
  7. 7.
    Reinecke, M., Betzler, D., Forssmann, W.G., Thorndyke, M., Askensten, U., Falkmer, S. (1987b) Electromicroscopical, immunohistochemical, immunocyto-chemical and biological evidence for the occurence of cardiac hormones (ANP/CDD) in chondrichthyes. Histochemistry 87: 531–538PubMedCrossRefGoogle Scholar
  8. 8.
    Schipp, R. (1987) General morphology and functional characteristics of the cephalopod circulatory system. An introduction. Experientia 43: 474–477CrossRefGoogle Scholar

Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1989

Authors and Affiliations

  • M. Reinecke
    • 1
  • H. Back
    • 1
  • P. Jacobs
    • 2
  • R. Schipp
    • 2
  1. 1.Department of Anatomy and Cell BiologyUniversity of HeidelbergGermany
  2. 2.Department of ZoologyUniversity of GiessenGermany

Personalised recommendations