Short-term follow-up of cryopreserved allograft valves and valved conduits from the CryoLife clinical registry

  • Robert T. McNally
  • A. E. Heacox
  • K. G. M. Brockbank
Conference paper


CryoLife, a laboratory which specializes in the cryopreservation of human tissues for transplant, first started preserving valves in Autumn 1984. The hearts which arrive at CryoLife are currently procured by 144 independent organ and tissue procurement agencies in the United States, Canada and West Germany. This procured tissue is preserved by CryoLife as a service to those agencies or doctors to whom the tissue has been assigned. In order to assure consistent quality control, all agencies follow the guidelines printed in the CryoLife (3) heart valve procurement protocol. The protocol provides information for screening for infectious diseases and diseases of the heart which would render the tissue less than ideal. For instance, when the required serum sample is returned by the procuring group, tests are performed for HIV (AIDS), hepatitis, syphilis and CMV (cytomegalovirus). These internal tests are compared with pretest data which accompanies the organ and are used as a double check to confirm that the possibility of transmission of these diseases is eliminated. The donor form also contains a detailed medical history of the donor, which helps eliminate donors of questionable standards or with a history of cardiac complications. The protocol also contains the precise details for the surgical extraction of the heart so that uniformity amongst participating centres is maintained.


Aortic Valve Heart Valve Valve Conduit Allograft Valve Ventricular Outflow Tract Reconstruction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Angell WW, De Lanerole P, Shumway NE (1973) Valve replacement: Present status of homograft valves. Prog Cardiovasc Dis 15: 589PubMedCrossRefGoogle Scholar
  2. 2.
    Broekbank KGM, Bank HL (1987) Measurement of post cryopreservation viability. J Cardiac Surg Suppl II: 145Google Scholar
  3. 3.
    CryoLife Inc. Clinical Program 101 allograft heart valves (1987) CryoLife Inc., Marietta, Georgia, U.S.A.Google Scholar
  4. 4.
    Kirklin JW, Barratt-Boyes BG (1986) Cardiac surgery. John Wiley, New York. Chapter 12: Aortic valve disease, p 373–429Google Scholar
  5. 5.
    Lockey E, Al-Jenabi N, Gonzales-Lavin L, Ross DN (1972) A method of sterilizing and preserving fresh allograft heart valves. Thorax 27: 398PubMedCrossRefGoogle Scholar
  6. 6.
    O’Brien MF, Stafford G, Gardner M, Phlner P, McGiffm D, Johnston N, Brosnan A, Duffy P (1987) The viable cryopreserved allograft aortic valve. J Cardiac Surg Suppl 2: 153–157Google Scholar
  7. 7.
    Ross D, Martelli M (1979) Allograft and autograft valves used for aortic valve replacement, In: Ionescu M (ed) Tissue heart valves. Butterworth, London, pp 127–172Google Scholar
  8. 8.
    van der Kamp AWM, Nuata J (1979) Fibroblast function and the maintenance of aortic valve matrix. Cardiovasc Res 13: 167PubMedCrossRefGoogle Scholar
  9. 9.
    van der Famp AWM, Visser WJ, van Dongen JM, et al (1981) Preservation of aortic heart valves with maintenance of cell viability. J Surg Res 30: 47CrossRefGoogle Scholar
  10. 10.
    Watts LK, Duffy P, Field RB, Stafford EG, O’Brien MF (1976) Establishment of a viable homograft cardiac valve bank: A rapid method of determining homograft viability. Ann Thorac Surg 21: 230PubMedCrossRefGoogle Scholar
  11. 11.
    Yankah AC, Randzio G, Wottge HU, Bernard A (1985) Factors influencing endothelial cell viability during procurement and preservation of valve allografts. In: Theide A, Deltz E, En-gemann R, Hamelmann H (eds) Microsurgical models in rats for transplantation research. Springer-Verlag, Berlin, p 107CrossRefGoogle Scholar
  12. 12.
    Yankah AC, Wottge HU, Muller-Hermelink HK, Feller AC, Large P, Wessel U, Dreyer H, Bern-hard A, Muller-Ruchholtz W (1987) Transplantation of aortic and pulmonary allografts, enhanced viability of endothelial cells by cryopreservation, importance of histocompatibility. J Cardiac Surg Suppl 2: 209–220Google Scholar

Copyright information

© Dr. Dietrich Steinkopff Verlag GmbH & Co. KG, Darmstadt 1988

Authors and Affiliations

  • Robert T. McNally
    • 2
  • A. E. Heacox
    • 1
  • K. G. M. Brockbank
    • 1
  1. 1.CryoLife Inc.MariettaUSA
  2. 2.CryoLife, Inc.MariettaUSA

Personalised recommendations