Femtosecond Dynamics, Two Dimensional Infrared Spectroscopy and Echoes of Protein Vibrations

  • Robin M. Hochstrasser
  • Peter Hamm
  • Manho Lim
Conference paper
Part of the Springer Series in Chemical Physics book series (CHEMICAL, volume 63)


Two approaches that use femtosecond infrared pulses to examine protein dynamics by means of nonlinear IR spectroscopy are described. In the first we dissect the featureless peptide amide-I band and introduce two-dimensional IR spectra [1]. In the second, the correlation function of the frequency fluctuations of a vibrational mode in the active site of carbonic anhydrase and heme pocket of hemoglobin is determined by three-pulse IR photon echoes [2]. These experiments permit the study of ultrafast dynamics without the requirement of chromophores.


Vibrational Relaxation Heme Pocket Zinc Cation Bovine Pancreatic Trypsin Inhibitor Photon Echo 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Robin M. Hochstrasser
    • 1
  • Peter Hamm
    • 1
  • Manho Lim
    • 1
  1. 1.Department of ChemistryUniversity of PennsylvaniaPhiladelphiaUSA

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