Abstract
A thermostated, stirred reactor of 14 mL volume with integrated pH-probe and control, mass spectrometer membrane probe and dosimeters was constructed. It allowed the measurement of gas exchange rates in anaerobic and aerobic systems together with the acid consumption or production rates. The accumulation of gases can be measured very sensitively, allowing short term measurements. This is particularly interesting for slowly growing organisms. Because of the small volume, studies with isotopes (e.g., 13C, 2H) become affordable. Kinetic experiments can be batch, continuous or fed-batch with stepwise or continuous feeding. Acetate and formate kinetics in mixed cultures were studied and kinetic models could be developed. In cultures of mammalian cells producing monoclonal antibodies, oxygen uptake and CO2 production rates were measured.
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© 1998 Springer-Verlag Berlin Heidelberg
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Heinzle, E., Meyer, B., Oezemre, A., Dunn, I.J. (1998). A Microreactor with On-line Mass Spectrometry for the Investigation of Biological Kinetics. In: Ehrfeld, W. (eds) Microreaction Technology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72076-5_31
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DOI: https://doi.org/10.1007/978-3-642-72076-5_31
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