Abstract
Haploid plants have the gametophytic number of chromosomes. They can be derived by several different ways. Haploids via twin seedlings can spontaneously occur in nature. Maternal haploids may arise from certain interspecific crosses followed by the elimination of chromosomes from the pollen donor during embryo development. By using in vitro culture techniques, haploid plants can also be regenerated from cultured anthers, microspores and ovaries. In this manner, both androgenetic (derived from microspores or immature pollen) and gynogenetic (derived from unfertilized egg cells) haploid tissue cultures and plants have been obtained in many different plant species. Gametes are the most convenient material for the reproducible production of haploids. Since normally in plants a larger number of haploid cells is concentrated in the anthers, which contain microspores or pollen, androgenesis theoretically represents the more efficient option. Therefore the techniques of anther culture and microspore culture have found wide application for the production of haploids, among others in several cereals and other grasses (Guha and Maheshwari 1964; Nitsch and Nitsch 1969; Nitsch 1977; Reinert and Bajaj 1977; Wenzel and Foroughi-Wehr 1984).
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Spangenberg, G., Wang, ZY., Potrykus, I. (1998). Anther Culture and Production of Haploids. In: Biotechnology in Forage and Turf Grass Improvement. Monographs on Theoretical and Applied Genetics, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72051-2_4
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DOI: https://doi.org/10.1007/978-3-642-72051-2_4
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