Short-Term Storage

  • Jeanne Dijkstra
  • Cees P. de Jager
Part of the Springer Lab Manual book series (SLM)


When virus samples collected in one country have to be transported by air to another country, measures should be taken to keep them in good condition for a number of days. The following two procedures described by Alhubashi et al. (1987) have been found to be satisfactory.


Calcium Chloride Plant Virus Leaf Material Blotting Paper Plant Virus Disease 
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  1. Alhubashi AA, Walkey DGA, Webb MJW, Bolland CJ, Cook AA (1987) A survey of horticultural plant virus diseases in the Yemen Arab Republic. FAO Plant Protection Bull 35: 135–143Google Scholar
  2. Bos L (1969) Experiences with a collection of plant viruses in leaf material dried and stored over calcium chloride, and a discussion of literature on virus preservation. Mededelingen Rijksfakulteit Landbouwwetenschappen Gent 34: 875–878Google Scholar
  3. Bos L, Benetti MP (1979) Direct electron microscopy and serology with plant viruses in leaf material dried and stored over calcium chloride. Neth J Plant Pathol 85: 241–251CrossRefGoogle Scholar
  4. Hollings M, Stone OW (1970) The long-term survival of some plant viruses preserved by lyophilization. Ann Appl Biol 65: 411–418CrossRefGoogle Scholar
  5. McKinney HH (1953) Plant-virus type culture collections. Ann NY Acad Sci 56: 615–620PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jeanne Dijkstra
    • 1
  • Cees P. de Jager
    • 1
  1. 1.Department of VirologyWageningen Agricultural UniversityWageningenThe Netherlands

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