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Analysis of Specific Protein-DNA Interactions

  • Indrikis Muiznieks
  • Nils Rostoks
Part of the Springer Lab Manual book series (SLM)

Abstract

The central issue in the regulation of genome functions is the mechanism of sequence-specific protein-nucleic acid interactions. Gene expression, replication, recombination and DNA condensation in chromatin are steered by binding of regulatory protein ligands to specific sites in DNA. Numerous methods have been developed to study protein-DNA interactions. In this chapter we discuss two widely used and straightforward approaches to address this problem.

Keywords

Electrophoretic Mobility Shift Assay Calf Intestinal Alkaline Phosphatase Klenow Enzyme Saran Wrap Footprinting Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Indrikis Muiznieks
    • 1
  • Nils Rostoks
  1. 1.Faculty of BiologyUniversity of LatviaRigaLatvia

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