Analysis of Specific Protein-DNA Interactions
The central issue in the regulation of genome functions is the mechanism of sequence-specific protein-nucleic acid interactions. Gene expression, replication, recombination and DNA condensation in chromatin are steered by binding of regulatory protein ligands to specific sites in DNA. Numerous methods have been developed to study protein-DNA interactions. In this chapter we discuss two widely used and straightforward approaches to address this problem.
KeywordsElectrophoretic Mobility Shift Assay Calf Intestinal Alkaline Phosphatase Klenow Enzyme Saran Wrap Footprinting Assay
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