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Monoclonal Antibodies Against Gangliosides

  • L. Svennerholm
Conference paper
Part of the NATO ASI Series book series (volume 7)

Abstract

A large number of murine monoclonal antibodies that react with gangliosides have been produced in the last few years. Most of them have been elicited by immunization with complex antigen mixtures, whole cells or tissue extracts, but recently some have been produced with pure gangliosides. Antibodies directed against carbohydrate determinants react with the sugar sequence linked either to lipid or peptide/protein. Since the gangliosides can be purified to homogeneity they are better suited as immunogens than glycoproteins, which often show microheterogeneity. Furthermore, carbohydrate antigens in glycoproteins are poorly immunogenic. Gangliosides are also more suitable than glycoproteins for use in establishing the epitope specificity (1). The antigenic determinant of monoclonal antibody 19-9 was determined with a ganglioside extract of the colon cancer used for the immunization, but the reactive antigen in the sera of cancer patients proved to be a mucin (2).

Keywords

Murine Monoclonal Antibody Human Glioma Cell Line Monoclonal Antibody 18B8 Sugar Sequence Ganglioside Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • L. Svennerholm
    • 1
  1. 1.Department of Psychiatry and NeurochemistryGothenburg University St. Jörgen HospitalHisings BackaSweden

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