Monoclonal Antibodies Against Gangliosides
A large number of murine monoclonal antibodies that react with gangliosides have been produced in the last few years. Most of them have been elicited by immunization with complex antigen mixtures, whole cells or tissue extracts, but recently some have been produced with pure gangliosides. Antibodies directed against carbohydrate determinants react with the sugar sequence linked either to lipid or peptide/protein. Since the gangliosides can be purified to homogeneity they are better suited as immunogens than glycoproteins, which often show microheterogeneity. Furthermore, carbohydrate antigens in glycoproteins are poorly immunogenic. Gangliosides are also more suitable than glycoproteins for use in establishing the epitope specificity (1). The antigenic determinant of monoclonal antibody 19-9 was determined with a ganglioside extract of the colon cancer used for the immunization, but the reactive antigen in the sera of cancer patients proved to be a mucin (2).
KeywordsCarbohydrate Adenocarcinoma Enzymatic Degradation Oligosaccharide Neuroblastoma
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