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Gangliosides and Modulation of Neuronal Functions pp 167–177Cite as

Up-To-Date Chromatography of Gangliosides

  • Conference paper

Part of the book series: NATO ASI Series ((ASIH,volume 7))

Abstract

The incomplete extraction of total gangliosides from biological materials has long been a source of considerable variation in quantitative studies. Since gangliosides are involved in strong interactions with other components in membranes of both ionic and hydrophobic nature as well as due to hydrogen bonding, mixed organic solvents containing water are required for their quantitative extraction (1,2). Taking advantage of the water- soluble nature of gangliosides, two-phase partitioning has widely been employed to separate gangliosides from most other lipids (3). In this partitioning, many of the less polar species, such as GM4 and GM3, are known to be lost. Recently, to overcome this loss, a new partitioning solvent system, diisopropylether/n-butanol/50 mM aqueous NaCl (6:4:5, by vol), was reported (4). Another quantitative method based on a different principle is a procedure using, sequentially, a DEAE-resin column, mild base treatment, desalting, and a silica gel column (5,6). This procedure has now become popular. Byrne et al. (7) reported the further improvement of this method by including an additional step using a Sephadex LH-20 column eluted with 0.05N HCl.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo, 173, Japan

    S. Ando, H. Waki & K. Kon

  2. John F. Kennedy Institute, Department of Neurology, Johns Hopkins University, Baltimore, MD, 21205, USA

    Y. Kishimoto

Authors
  1. S. Ando
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  2. H. Waki
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  3. K. Kon
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  4. Y. Kishimoto
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Editor information

Editors and Affiliations

  1. Zoological Institute, University of Stuttgart-Hohenheim, Garbenstr. 30, 7000, Stuttgart 70, Germany

    Hinrich Rahmann

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© 1987 Springer-Verlag Berlin Heidelberg

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Ando, S., Waki, H., Kon, K., Kishimoto, Y. (1987). Up-To-Date Chromatography of Gangliosides. In: Rahmann, H. (eds) Gangliosides and Modulation of Neuronal Functions. NATO ASI Series, vol 7. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71932-5_11

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  • DOI: https://doi.org/10.1007/978-3-642-71932-5_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-71934-9

  • Online ISBN: 978-3-642-71932-5

  • eBook Packages: Springer Book Archive

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