Long-Term Murine Hemopoietic Cultures as Model Systems for Analysis of B Lymphocyte Differentiation
The development of B lymphocytes in the bone marrow proceeds in parallel with that of other hemopoietic lineages (Rosse 1976). Phenotypic analysis using antibodies that detect immunoglobulin (Ig; Osmond and Nossal 1974; Abney et al. 1978; Landreth et al. 1981; Rahal and Osmond 1984) and other nonIg cellular determinants (reviewed by Scheid et al. 1982) has identified cells in the B-lymphocyte lineage and contributed greatly to the delineation of stages of that process. However, these studies have not always identified the earliest stages of B lymphopoiesis, or permitted regulatory events that occur during B-cell differentiation to be dissected. In order to facilitate such investigations, considerable efforts have been made to develop in vitro systems that permit all phases of primary B-cell development to be studied under controlled conditions.
KeywordsFetal Liver Bone Marrow Culture Hemopoietic Cell Adherent Layer Fresh Bone Marrow
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