Long-Term Murine Hemopoietic Cultures as Model Systems for Analysis of B Lymphocyte Differentiation
The development of B lymphocytes in the bone marrow proceeds in parallel with that of other hemopoietic lineages (Rosse 1976). Phenotypic analysis using antibodies that detect immunoglobulin (Ig; Osmond and Nossal 1974; Abney et al. 1978; Landreth et al. 1981; Rahal and Osmond 1984) and other nonIg cellular determinants (reviewed by Scheid et al. 1982) has identified cells in the B-lymphocyte lineage and contributed greatly to the delineation of stages of that process. However, these studies have not always identified the earliest stages of B lymphopoiesis, or permitted regulatory events that occur during B-cell differentiation to be dissected. In order to facilitate such investigations, considerable efforts have been made to develop in vitro systems that permit all phases of primary B-cell development to be studied under controlled conditions.
KeywordsAgar Leukemia Corticosteroid Sedimentation Nylon
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- Aspinall R, Owen JJT (1983) An investigation into the B lymphopoietic capacity of long-term bone marrow cultures. Immunilogy 48: 9–15Google Scholar
- Denis KA, Witte ON (1986) In vitro development of B lymphocyte from long-term cultured precursor cells. Proc Natl Acad Sci USA (to be published)Google Scholar
- Phillips RA (1985) Comparison of different assays for multipotent haemopoietic stem cells. In: Ford RJ, Maizel AL (eds) Mediators in cell growth and differentiation. Raven, New York, p 135Google Scholar
- Phillips RA, Bosma M, Dorshkind K (1984) Reconstitution of immune-deficient mice with cells from long-term bone marrow cultures. In: Wright DG, Greenberger JS (eds), Long-term bone marrow culture. Liss, New York, p 309Google Scholar
- Rosenberg N, Baltimore D (1980) Abelson virus. In: Klein G (ed) Viral oncology, Raven, New York, p 187Google Scholar
- Tavassoli M (1982) Ultrastructural and radiobiological characterization of stromal cells in continuous, long-term marrow cell line. In: Johari O (ed) Scanning electron mcirosocpy. SEM, AMF O’Hare, Chicago, p 349Google Scholar