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Long-Term Murine Hemopoietic Cultures as Model Systems for Analysis of B Lymphocyte Differentiation

  • Conference paper
Differentiation of B Lymphocytes

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 135))

Abstract

The development of B lymphocytes in the bone marrow proceeds in parallel with that of other hemopoietic lineages (Rosse 1976). Phenotypic analysis using antibodies that detect immunoglobulin (Ig; Osmond and Nossal 1974; Abney et al. 1978; Landreth et al. 1981; Rahal and Osmond 1984) and other nonIg cellular determinants (reviewed by Scheid et al. 1982) has identified cells in the B-lymphocyte lineage and contributed greatly to the delineation of stages of that process. However, these studies have not always identified the earliest stages of B lymphopoiesis, or permitted regulatory events that occur during B-cell differentiation to be dissected. In order to facilitate such investigations, considerable efforts have been made to develop in vitro systems that permit all phases of primary B-cell development to be studied under controlled conditions.

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References

  • Abney ER, Cooper MD, Kearney JF, Lawton AR, Parkhouse RME (1978) Sequential expression of immunoglobulin in developing mouse B lymphocytes: a systematic survey that suggests a model for the generation of immunoglobulin isotype diversity. J Immunol 120: 2041–2049

    PubMed  CAS  Google Scholar 

  • Allen TD, Dexter TM (1976) Cellular interrelationships during in vitro granulopoiesis. Differentiation 6: 191–194

    Article  PubMed  CAS  Google Scholar 

  • Aspinall R, Owen JJT (1983) An investigation into the B lymphopoietic capacity of long-term bone marrow cultures. Immunilogy 48: 9–15

    CAS  Google Scholar 

  • Boettiger D, Anderson S, Dexter TM (1985) Effect of SRC on hemopoietic pronator cells: increased self-renewal without leukemia. Cell 36: 763–773

    Article  Google Scholar 

  • Bosma GC, Custer RP, Bosma MJ (1983) A severe combined immunodeficiency mutation in the mouse. Nature 301: 527–530

    Article  PubMed  CAS  Google Scholar 

  • Cass CE, Lowe JK, Machak JM, Henderson JF (1977) Biological effects of inhibition of guanine nucleotide synthesis by mycophenolic acid in cultured neurblastoma cells. Cancer Res 37: 3314–3320

    PubMed  CAS  Google Scholar 

  • Denis KA, Witte ON (1986) In vitro development of B lymphocyte from long-term cultured precursor cells. Proc Natl Acad Sci USA (to be published)

    Google Scholar 

  • Denis KA, Treiman LJ, St Claire JI, Witte ON (1984) Long-term cultures of murine fetal liver retain very early B lymphoid phenotype. J Exp Med 160: 1087–1101

    Article  PubMed  CAS  Google Scholar 

  • Dexter TM, Spooncer E (1978) Loss of immunoreactivity in long-term bone marrow culture. Nature 275: 135–136

    Article  PubMed  CAS  Google Scholar 

  • Dexter TM, Allen TD, Lajtha LG (1977a) Conditions controlling the proliferation of haemopoietic stem cells in vitro. J Cell Physiol 91: 335–344

    Article  PubMed  CAS  Google Scholar 

  • Dexter TM, Moore MAS, Sheridan APC (1977b) Maintenance of hemopoietic stem cells and production of differentiated progeny in allogeneic and semiallogeneic bone marrow chimeras in vitro. J Exp Med 145: 1612–1616

    Article  PubMed  CAS  Google Scholar 

  • Dick JE, Magli MC, Huszar D, Phillips RA, Bernstein A (1985) Introduction of a selectable gene into primitive stem cells capable of long–term reconstitution of the hemopoietic system of W/W mice. Cell 42: 71–79

    Article  PubMed  CAS  Google Scholar 

  • Dorshkind K (1986) In vitro differentiation of B lymphocytes from primitive hemopoietic precursors present in long-term bone marrow cultures. J Immunol 136: 422–429

    PubMed  CAS  Google Scholar 

  • Dorshkind K, Phillips RA (1982) Maturational state of lymphoid cells in long-term bone marrow cultures. J Immunol 129: 2444–2450

    PubMed  CAS  Google Scholar 

  • Dorshkind K, Phillips RA (1983) Characterization of early B lymphocyte precursors present in long-term bone marrow cultures. J Immunol 131: 2240–2245

    PubMed  CAS  Google Scholar 

  • Dorshkind K, Keller GM, Phillips RA, Miller RG, Bosma GC, O’Toole M, Bosma MJ (1984) Functional status of cells from lymphomyeloid tissues in mice with severe combined immunodeficiency disease. J Immunol 132: 1804–1808

    PubMed  CAS  Google Scholar 

  • Dorshkind K, Schouest L, Fletcher WH (1985) Morphologic analysis of long-term bone marrow cultures that support B lymphopoiesis or myelopoiesis. Cell Tissue Res 239: 375–382

    Article  PubMed  CAS  Google Scholar 

  • Dorshkind K, Johnson A, Collins L, Keller GM, Philips RA (1986) Generation of purified stromal cell cultures that support lymphoid and myeloid precursors. J Immunol Methods 89: 37–47

    Article  PubMed  CAS  Google Scholar 

  • Fulop GM, Osmond DG (1983) Regulation of bone marrow lymphocyte production. III. Increased production of B and non-B lymphocytes after administering systemic antigens. Cell Immunol 75: 80–90

    Article  PubMed  CAS  Google Scholar 

  • Johnson A, Dorshkind K (1986) Stromal cells in myeloid and lymphoid long-term bone marrow cultures can support multiple hemopoietic lineages and modulate their production of hemopoietic growth factors. Blood 68: 1348–1354

    PubMed  CAS  Google Scholar 

  • Jones-Villeneuve E, Phillips RA (1980) Potentials for lymphoid differentiation by cells from long-term cultures of bone marrow. Exp Hematol 8: 65–76

    PubMed  CAS  Google Scholar 

  • Joyner A, Keller G, Phillips RA, Bernstein A (1983) Retrovirus mediated transfer of a bacterial gene into mouse hematopoietic progenitor cells. Nature 305: 206–208

    Article  Google Scholar 

  • Jyonouchi H, Kincade PW (1984) Precocious and enhanced functional maturation of B lineage cells in New Zealand black mice during embryonic development. J Exp Med 159: 1277–1282

    Article  PubMed  CAS  Google Scholar 

  • Keller G, Paige C, Gilboa E, Wagner EF (1985) Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors. Nature 318: 149–154

    Article  PubMed  CAS  Google Scholar 

  • Keller GM, Johnson GR, Phillips RA (1983) Hemopoiesis in spleen and bone marrow cultures. J Cell Physiol 116: 7–15

    Article  PubMed  CAS  Google Scholar 

  • Kincade PW (1977) Defective colony formation by B lymphocytes from CBA/N and C3H/HeJ mice. J Exp Med 145: 249–263

    Article  PubMed  CAS  Google Scholar 

  • Kincade PW, Lee G, Paige CJ, Scheid MP (1981) Cellular interactions affecting the maturation of murine B lymphocyte precursors in vitro. J Immunol 127: 255–260

    PubMed  CAS  Google Scholar 

  • Kurland JI, Ziegler SF, Witte ON (1984) Long-term cultured B lymphocytes and their precursors reconstitute the B-lymphocyte lineage in vivo. Proc Natl Acad Sci USA 81: 7754–7558

    Article  Google Scholar 

  • Landreth KS, Rosse C, Clagett J (1981) Myelogenous production and maturation of B lymphocytes in the mouse. J Immunol 127: 2027–2034

    PubMed  CAS  Google Scholar 

  • Landreth KS, Kincade PW, Lee G, Medlock ES (1983) Phenotypic and functional characterization of murine B lymphocyte precursors isolated from fetal and adult tissues. J Immunol 131: 572–580

    PubMed  CAS  Google Scholar 

  • Landreth KS, Engelhard D, Bear MH, Kincade PW, Kapoor W, Good RA (1985) Regulation of human B lymphopoiesis: Effect of urinary activity associated with cyclic neutropenia. J Immunol 134: 2305–2309

    PubMed  CAS  Google Scholar 

  • Lanotte M, Scott D, Dexter TM, Allen TD (1982) Clonal preadipocyte cell lines with different phenotypes derived from murine marrow stroma. Factors influencing growth and adipogenesis in vitro. J Cell Physiol 111: 177–186

    Article  PubMed  CAS  Google Scholar 

  • Lau CY, Melchers F, Miller RG, Phillips RA (1979) In vitro differentiation of B lymphocytes from pre-B cells. J Immunol 122: 1273–1277

    PubMed  CAS  Google Scholar 

  • Lichtman MA (1981) The ultrastrueture of the hemopoietic environment of the marrow: a review. Exp Hematol 9: 391–420

    PubMed  CAS  Google Scholar 

  • Mauch P, Greenberger JS, Botnick LE, Hannon EC, Hellman S (1980) Evidence for structured variation in self–renewal capacity within long-term bone marrow cultures. Proc Natl Acad Sci USA 77: 2927–2930

    Article  PubMed  CAS  Google Scholar 

  • Melchers F (1977) B lymphocyte development in fetal liver. I. Development of reactivities to B cell mitogens in vivo and in vitro. Eur J Immunol 7: 476–481

    Article  PubMed  CAS  Google Scholar 

  • Mongini PKA, Paul WE, Metcalf ES (1983) IgG subclass, IgE, and IgA anti-trinitrophenyl antibody production within trinitrophenyl-Ficoll-responsive B cell clones. Evidence in support of three distinct switching pathways. J Exp Med 157: 69–85

    Article  PubMed  CAS  Google Scholar 

  • Nagasawa R, Kanagawa O, Tittle TV, Chiller JM (1985) In vivo maturation of in vitro derived pre-B cells. J Immunol 135: 965–972

    PubMed  CAS  Google Scholar 

  • Nishikawa S, Kina T, Amagai T, Katsura Y (1985) Early B cell precursors in long-term bone marrow culture: selective development in the bone marrow of irradiated recipients. Eur J Immunol 15: 696–701

    Article  PubMed  CAS  Google Scholar 

  • Osmond DG, Nossal GJV (1974) Differentiation of lymphocytes in mouse bone marrow. II. Kinetics of maturation and renewal of antiglobulin-binding cells studied by double labelling. Cell Immunol 13: 132–145

    Article  PubMed  CAS  Google Scholar 

  • Owen JJT, Cooper MD, Raff MC (1974) In vitro generation of B lymphocytes in mouse fetal liver; a mammalian bursal equivalent. Nature 249: 361–363

    Article  PubMed  CAS  Google Scholar 

  • Paige CJ (1983) Surface immunoglobulin-negative B-cell precursors detected by formation of antibody-secreting colonies in agar. Nature 302: 711–713

    Article  PubMed  CAS  Google Scholar 

  • Paige CJ, Gisler RH, McKearn JP, Iscove NN (1984) Differentiation of murine B cell precursors in agar culture. Frequency, surface marker analysis and requirements for growth of clonable pre-B cells. Eur J Immunol 17: 979–987

    Article  Google Scholar 

  • Palacios R, Steinmetz M (1985) IL-3 dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes. Cell 41: 727–734

    Article  PubMed  CAS  Google Scholar 

  • Palacios R, Henson G, Steinmetz M, McKearn J (1984) Interleukin 3 supports growth of mouse Pre-B cells clones in vitro. Nature 309: 126–134

    Article  PubMed  CAS  Google Scholar 

  • Phillips RA (1980) Enhanced lymphoid and decreased myeloid reconstituting ability of stem cells from long-term cultures of mouse bone marrow. J Supramol Struct 14: 77–83

    Article  PubMed  CAS  Google Scholar 

  • Phillips RA (1985) Comparison of different assays for multipotent haemopoietic stem cells. In: Ford RJ, Maizel AL (eds) Mediators in cell growth and differentiation. Raven, New York, p 135

    Google Scholar 

  • Phillips RA, Bosma M, Dorshkind K (1984) Reconstitution of immune-deficient mice with cells from long-term bone marrow cultures. In: Wright DG, Greenberger JS (eds), Long-term bone marrow culture. Liss, New York, p 309

    Google Scholar 

  • Rahal MD, Osmond DG (1984) Heterogeneity of bone marrow lymphocytes: Radioautographic detection of pre-B cells bearing cytoplasmic μ chains and of B and T lymphocytes and characterization of null lymphoid cells. Cell Immunol 87: 379–388

    Article  PubMed  CAS  Google Scholar 

  • Rosenberg N, Baltimore D (1980) Abelson virus. In: Klein G (ed) Viral oncology, Raven, New York, p 187

    Google Scholar 

  • Rosse C (1976) Small lymphocyte and transitional cell populations of the bone marrow; their role in the mediation of immune and hemopoietic progenitor cell functions. Int Rev Cytol 45: 155–290

    Article  PubMed  CAS  Google Scholar 

  • Scheid MP, Landreth KS, Tung JS, Kincade PW (1982) Preferential but nonexclusive expression of macromolecular antigens on B-lineage cells. Immunol Rev 69: 141–159

    Article  PubMed  CAS  Google Scholar 

  • Schrader JW, Schrader S (1978) In vitro studies on lymphocyte differentiation. I. Long-term in vitro culture of cells giving rise to functional lymphocytes in irradiated mice. J Exp Med 148: 823–828

    Article  PubMed  CAS  Google Scholar 

  • Schrader JW, Goldschneider I, Bollum FJ, Schrader S (1979) In vitro studies in lymphocyte differentiation. II. Generation of terminal deoxynucleotidyl transferase-positive cells in long-term cultures of mouse bone marrow. J Immunol 122: 2337–2339

    PubMed  CAS  Google Scholar 

  • Song ZX, Quesenberry PJ (1984) Radioresistant murine marrow stromal cells: a morphologic and functional characterization. Exp Hematol 12: 523–533

    PubMed  CAS  Google Scholar 

  • Song ZX, Shadduck RK, Innes DJ, Waheed A, Quesenberry PJ (1985) Hematopoietic factor production by a cell line (TC-1) derived from adherent murine marrow cells. Blood 66: 273–281

    PubMed  CAS  Google Scholar 

  • Tavassoli M (1982) Ultrastructural and radiobiological characterization of stromal cells in continuous, long-term marrow cell line. In: Johari O (ed) Scanning electron mcirosocpy. SEM, AMF O’Hare, Chicago, p 349

    Google Scholar 

  • Van der Putten H, Botteri FM, Miller AD, Rosenfeld MG, Fan H, Evans RM, Verma IM (1985) Efficient insertion of genes into the mouse germ-line via retroviral vectors. Proc Natl Acad Sci USA 82: 6148–6152

    Article  PubMed  Google Scholar 

  • Weiss L (1970) Transmural cellular passage in vascular sinuses of rat bone marrow. Blood 36: 189–208

    PubMed  CAS  Google Scholar 

  • Whitlock CA, Witte ON (1982) Long-term culture of B lymphocytes and their precursors from murine bone marrow. Proc Natl Acad Sci USA 79: 3608–3612

    Article  PubMed  CAS  Google Scholar 

  • Whitlock CA, Ziegler SF, Treiman LJ, Stafford JI, Witte ON (1983) Differentiation of cloned populations of immature B cells after transformation with Abelson murine leukemia virus. Cell 32: 903–911

    Article  PubMed  CAS  Google Scholar 

  • Whitlock CA, Robertson D, Witte ON (1984) Murine B cell lymphopoiesis in long-term culture. J Immunol Methods 16: 353–369

    Article  Google Scholar 

  • Williams N, Jackson H, Sheridan APC, Murphy MJ, Eisle A, Moore MAS (1978) Regulation of megakaryopoiesis in long-term murine bone marrow cultures. Blood 51: 245–255

    PubMed  CAS  Google Scholar 

  • Williams DA, Lemischka IR, Nathans DG, Mulligan RC (1984) Induction of new genetic material into pluripotent haemopoietic stem cells of the mouse. Nature 310: 476–480

    Article  PubMed  CAS  Google Scholar 

  • Wolf NS, Trentin JJ (1968) Hemopoietic colony studies. V. Effect of hemopoietic organ stroma on differentiation. J Exp Med 127: 205–214

    Article  CAS  Google Scholar 

  • Ziegler SF, Treiman LJ, Witte ON (1984) K gene diversity among the clonal progeny of pre-B lymphocytes. Proc Natl Acad Sci USA 81: 1529–1533

    Article  PubMed  CAS  Google Scholar 

  • Zipori D, Duskin D, Tamir M, Argaman A, Toledo J, Malik Z (1985) Cultured mouse marrow stromal cell lines. II. Distinct subtypes differing in morphology, collagen types, myelopoietic factors, and leukemic cell growth modulating activities. J Cell Physiol 122: 81–90

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Biomedical Sciences, University of California, Riverside, CA, 92521-0121, USA

    K. Dorshkind

  2. Department of Microbiology and Molecular Biology Institute, University Of California, Los Angeles, CA, 90024, USA

    O. N. Witte

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  1. K. Dorshkind
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  2. O. N. Witte
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Editors and Affiliations

  1. Basel Institute for Immunology, Grenzacherstrasse 487, CH-4058, Basel, Switzerland

    Christopher J. Paige  & Roland H. Gisler  & 

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© 1987 Springer-Verlag Berlin Heidelberg

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Dorshkind, K., Witte, O.N. (1987). Long-Term Murine Hemopoietic Cultures as Model Systems for Analysis of B Lymphocyte Differentiation. In: Paige, C.J., Gisler, R.H. (eds) Differentiation of B Lymphocytes. Current Topics in Microbiology and Immunology, vol 135. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-71851-9_2

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  • DOI: https://doi.org/10.1007/978-3-642-71851-9_2

  • Publisher Name: Springer, Berlin, Heidelberg

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